FBXO45 expression levels Correlation with cancer severity by ZEB1 ubiquitin in non-small cell lung cancer

Abstract

The early diagnostic methods for Non-small cell lung cancer (NSCLC) are limited, lacking effective biomarkers, and the late stage surgery is difficult and has a high recurrence rate. This experiment want to know whether the effects of FBXO45 in arcinogenesis and metastasis of NSCLC. The up-regulation of FBXO45 expression in patients with NSCLC or cell lines were observed. FBXO45 gene promoted metastasis and Warburg effect, and reduced Ferroptosis of NSCLC. FBXO45 induced ZEB1 expression to promote Warburg effect and reduced Ferroptosis of NSCLC. Sh-FBXO45 reduced cancer growth of NSCLC in mice model. FBXO45 decreased the ubiquitination of ZEB1, leading to increased expression of ZEB1, which in turn promoted the Warburg effect and reduced Ferroptosis in NSCLC. In vivo imaging, Sh-FBXO45 also reduced ZEB1 expression levels of lung tissue in mice model. FBXO45 in NSCLC through activating the Warburg effect, and the inhibition of Ferroptosis of NSCLC by the suppression of ZEB1 ubiquitin, FBXO45 may be a potential therapeutic strategy for NSCLC.