In vitro stimulation with nontuberculous mycobacteria induced a stronger cytokine response in leukocytes isolated from individuals with latent tuberculosis compared to those isolated from active tuberculosis or cystic fibrosis patients

IF 2.8 3区医学 Q3 IMMUNOLOGY

Tuberculosis Pub Date : 2024-03-20 DOI:10.1016/j.tube.2024.102504

Hardis Rabe , Elisabeth Lönnermark , Ewa Johansson , Marita Gilljam , Bodil Jönsson

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引用次数: 0

Abstract

Mycobacterium tuberculosis and opportunistic environmental non-tuberculous mycobacteria (NTM) can cause severe infection. Why latent tuberculosis infection advances to active disease, and why some individuals with cystic fibrosis (CF) develop pulmonary infections with NTM is still poorly understood. The aim of this study was to investigate the effector function of peripheral blood mononuclear cells (PBMC) from individuals with active or latent tuberculosis, individuals with CF with or without pulmonary NTM-infection and healthy controls, by measuring cytokine response to in vitro stimulation with different species of NTMs. The cytokine concentrations of IL-17A, IL-22, IL-23, IL-10, IL12p70 and IFN-γ were measured in PBMC-culture supernatants after stimulation with NTMs. PBMCs from individuals with latent tuberculosis infection showed strong IL-17A, IL-22, and IFN-γ responses compared to individuals with active tuberculosis or CF. IL-10 production was low in both tuberculosis groups compared to the CF groups and controls. This study suggests that IL-17A and IL-22 might be important to keep tuberculosis in a latent phase and that individuals with CF with an ongoing NTM infection seem to have a low cytokine response.

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与分离自活动性肺结核或囊性纤维化患者的白细胞相比，分离自潜伏肺结核患者的白细胞受到非结核分枝杆菌体外刺激后会产生更强的细胞因子反应

结核分枝杆菌和机会性环境非结核分枝杆菌（NTM）可导致严重感染。为什么潜伏结核感染会发展为活动性疾病，为什么一些囊性纤维化（CF）患者会出现肺部非结核分枝杆菌感染，这些问题仍然鲜为人知。本研究旨在通过测量细胞因子对不同种类非结核菌体外刺激的反应，研究活动性或潜伏性肺结核患者、伴有或不伴有肺部非结核菌感染的 CF 患者以及健康对照组的外周血单核细胞（PBMC）的效应功能。在受到 NTMs 刺激后，测定了 PBMC 培养上清液中 IL-17A、IL-22、IL-23、IL-10、IL12p70 和 IFN-γ 的细胞因子浓度。与活动性肺结核或 CF 感染者相比，潜伏肺结核感染者的白细胞对 IL-17A、IL-22 和 IFN-γ 的反应强烈。与 CF 组和对照组相比，两组肺结核患者的 IL-10 产生量都很低。这项研究表明，IL-17A和IL-22对结核病的潜伏期可能很重要，而正在感染NTM的CF患者对细胞因子的反应似乎较低。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Tuberculosis 医学-呼吸系统

CiteScore

4.60

自引率

3.10%

发文量

审稿时长

49 days

期刊介绍： Tuberculosis is a speciality journal focusing on basic experimental research on tuberculosis, notably on bacteriological, immunological and pathogenesis aspects of the disease. The journal publishes original research and reviews on the host response and immunology of tuberculosis and the molecular biology, genetics and physiology of the organism, however discourages submissions with a meta-analytical focus (for example, articles based on searches of published articles in public electronic databases, especially where there is lack of evidence of the personal involvement of authors in the generation of such material). We do not publish Clinical Case-Studies. Areas on which submissions are welcomed include: -Clinical TrialsDiagnostics- Antimicrobial resistance- Immunology- Leprosy- Microbiology, including microbial physiology- Molecular epidemiology- Non-tuberculous Mycobacteria- Pathogenesis- Pathology- Vaccine development. This Journal does not accept case-reports. The resurgence of interest in tuberculosis has accelerated the pace of relevant research and Tuberculosis has grown with it, as the only journal dedicated to experimental biomedical research in tuberculosis.