Detection of uric acid using colorimetric method based on iron-doped single-atom catalyst with peroxidase activity

Monatshefte für Chemie / Chemical Monthly Pub Date : 2024-03-20 DOI:10.1007/s00706-024-03199-z

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Abstract

Single-atom catalysts (SACs) have garnered considerable attention owing to their impeccable catalytic activity and unique ability to elucidate the active site. In this work, we synthesized iron-doped single-atom catalysts (Fe SACs) via a soft-template sacrificial approach, which exhibited peroxidase activity. Further investigations revealed that Fe SACs were capable of catalyzing the generation of highly reactive hydroxyl radicals (•OH) from hydrogen peroxide (H₂O₂), which facilitated the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) to oxTMB, resulting in a discernible color change from colorless to blue. According to the exceptional peroxidase activity of Fe SACs, we designed and fabricated a colorimetric sensor by combining uricase and TMB, achieving highly sensitive and selective detection of uric acid over a wide range of concentrations (0.1–50 μM), with a low detection limit of 0.039 μM (3σ/k). Finally, we demonstrated the feasibility of our sensor in detecting uric acid in real human urine samples, yielding satisfactory recoveries and low relative standard deviations.

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基于具有过氧化物酶活性的掺铁单原子催化剂的比色法检测尿酸

摘要单原子催化剂（SACs）因其无可挑剔的催化活性和阐明活性位点的独特能力而备受关注。在这项工作中，我们通过软模板牺牲法合成了掺铁单原子催化剂（Fe SACs），该催化剂具有过氧化物酶活性。进一步研究发现，铁单质原子催化剂能够催化过氧化氢（H2O2）生成高活性羟基自由基（-OH），从而促进 3,3',5,5'-四甲基联苯胺（TMB）氧化成 oxTMB，使颜色从无色变为蓝色。根据 Fe SACs 特殊的过氧化物酶活性，我们设计并制作了一种结合尿酸酶和 TMB 的比色传感器，实现了在较大浓度范围内（0.1-50 μM）对尿酸的高灵敏度和选择性检测，检测限低至 0.039 μM (3σ/k)。最后，我们证明了我们的传感器检测真实人体尿样中尿酸的可行性，回收率令人满意，相对标准偏差较低。图表摘要

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Monatshefte für Chemie / Chemical Monthly

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