{"title":"Cloning and expression analysis of Aerenchyma development related ShRBOH1 gene in Saussurea inversa Raab-Straube","authors":"","doi":"10.1007/s40415-024-00999-9","DOIUrl":null,"url":null,"abstract":"<h3>Abstract</h3> <p><em>Saussurea inversa</em> Raab-Straube is a typical alpine plant with well-developed aerenchyma, which is its most representative structural feature. Studying the formation and development of aerenchyma in <em>S. inversa</em> is of great theoretical significance for understanding the adaptive mechanisms of alpine plants to the environment. In this study, the aerenchyma development-related gene <em>ShRBOH1</em> (GenBank accession number OP727799) was cloned from <em>S. inversa</em> by RT-PCR with RACE technology. Sequence analysis revealed that the cDNA of <em>ShRBOH1</em> was 2158 bp long including an open reading frame of 2031 bp that encodes 676 amino acids. Subcellular localization prediction indicated that the ShRBOH1 protein is located in the cytoplasmic membrane, consistent with the localization of the NADPH protein. A comparison of the amino acid sequence homology showed that ShRBOH1 had higher similarity to CcRBOH1 of <em>Cynara cardunculus</em> L. The qRT-PCR analysis demonstrated that the <em>ShRBOH1</em> gene was expressed in roots, stems, and leaves with significantly lower expression level in stems compared to other tissues. The expression of the <em>ShRBOH1</em> gene was down-regulated under UV stress, up-regulated under cold stress, and initially up-regulated, then down-regulated, and finally up-regulated again under hypoxia stress. By comparing the changes in reactive oxygen species (ROS) content under the three types of stresses, we found that significant changes only occurred under hypoxic stress and were positively correlated with the expression of <em>ShRBOH1</em>. We hypothesize that <em>ShRBOH1</em> positively regulates the formation of aerenchyma through ROS mediation, suggesting that the aerenchyma is primarily an important structure for adapting to hypoxia stress in alpine environments.</p>","PeriodicalId":9140,"journal":{"name":"Brazilian Journal of Botany","volume":"20 1","pages":""},"PeriodicalIF":1.4000,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Brazilian Journal of Botany","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s40415-024-00999-9","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Saussurea inversa Raab-Straube is a typical alpine plant with well-developed aerenchyma, which is its most representative structural feature. Studying the formation and development of aerenchyma in S. inversa is of great theoretical significance for understanding the adaptive mechanisms of alpine plants to the environment. In this study, the aerenchyma development-related gene ShRBOH1 (GenBank accession number OP727799) was cloned from S. inversa by RT-PCR with RACE technology. Sequence analysis revealed that the cDNA of ShRBOH1 was 2158 bp long including an open reading frame of 2031 bp that encodes 676 amino acids. Subcellular localization prediction indicated that the ShRBOH1 protein is located in the cytoplasmic membrane, consistent with the localization of the NADPH protein. A comparison of the amino acid sequence homology showed that ShRBOH1 had higher similarity to CcRBOH1 of Cynara cardunculus L. The qRT-PCR analysis demonstrated that the ShRBOH1 gene was expressed in roots, stems, and leaves with significantly lower expression level in stems compared to other tissues. The expression of the ShRBOH1 gene was down-regulated under UV stress, up-regulated under cold stress, and initially up-regulated, then down-regulated, and finally up-regulated again under hypoxia stress. By comparing the changes in reactive oxygen species (ROS) content under the three types of stresses, we found that significant changes only occurred under hypoxic stress and were positively correlated with the expression of ShRBOH1. We hypothesize that ShRBOH1 positively regulates the formation of aerenchyma through ROS mediation, suggesting that the aerenchyma is primarily an important structure for adapting to hypoxia stress in alpine environments.
期刊介绍：
The Brazilian Journal of Botany is an international journal devoted to publishing a wide-range of research in plant sciences: biogeography, cytogenetics, ecology, economic botany, physiology and biochemistry, morphology and anatomy, molecular biology and diversity phycology, mycology, palynology, and systematics and phylogeny.
The journal considers for publications original articles, short communications, reviews, and letters to the editor.
Manuscripts describing new taxa based on morphological data only are suitable for submission; however information from multiple sources, such as ultrastructure, phytochemistry and molecular evidence are desirable.
Floristic inventories and checklists should include new and relevant information on other aspects, such as conservation strategies and biogeographic patterns.
The journal does not consider for publication submissions dealing exclusively with methods and protocols (including micropropagation) and biological activity of extracts with no detailed chemical analysis.
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