Antioxidant Activities and Phenolic Composition of Sarcococca saligna Leaves

IF 0.7 4区化学 Q4 CHEMISTRY, ORGANIC

Letters in Organic Chemistry Pub Date : 2024-03-18 DOI:10.2174/0115701786293349240229074809

Syed Mubashar Sabir, Shabnam Shahida, Alam Zeb, Syed Rizwan Abbas, Muhammad Usman Hameed, Abdallah Shanableh, Muhammad Imran Khan, Djamel Ghernaout, Ramzi Hadj Lajimi, Sarra Elgharbi, Malek Besbes, Noureddine Elboughdiri

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引用次数: 0

Abstract

: Sarcococca saligna (D. DON) Muel is a vital plant with several steroidal alkaloids and is effective against syphilis, liver diseases, gastrointestinal tract disease, infections, and inflammation. The present study aimed to investigate the in vitro antioxidant activities and phenolic profile of S. saligna. High-performance liquid chromatography (HPLC) analysis has shown the presence of twelve phenolics namely kaempferol-3-O-glucoside, proanthocyanidin B1, quercetin-3-malonylglucoside- 7-glucoside, kaempferol-3-O-sophorotioside, Isorhamnetin-3-O-glucoside-7-Orhamnoside, kaempferolhexoside, 4-O-Caffeoyl-5-O-p-coumaroylquinic acid, 3,4-Di-O-caffeoyl-5- O-feruloylquinic acid, 3,4-Di-O-feruloyl-5-O-caffeoylquinic acid, flavogalloyl-HHDP-gluconic acid (lagerstannin B), 3,4-di-O-caffeoylquinic acid and kaempferol-3-(caffeoyldiglucoside)-7-glucoside. The hot water extract showed the highest anti-lipid peroxidative activities. The IC50 value for 2,2- diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity was 71.5 ± 2.1 μg/mL. The extract could chelate the iron and possess reducing activities on phosphomolybenum assay. It is concluded that extracts of S. saligna are rich in antioxidants and contain essential phytochemicals. objective: The present study was aimed to investigate thein vitro antioxidant activities and phenolic profile of S. saligna. HPLC analysis has shown the presence of twelve phenolics namely kaempferol-3-O-glucoside, proanthocyanidin B1, quercetin-3-malonyl-glucoside-7-glucoside, kaempferol-3-O-sophorotioside,Isorhamnetin-3-O glucoside-7-O-rhamnoside,kaempferolhexoside, 4-O-Caffeoyl-5-O-p-coumaroylquinic acid, 3,4-Di-O-caffeoyl-5-O-feruloylquinic acid, 3,4-Di-O-feruloyl-5-O-caffeoylquinic acid, flavogalloyl-HHDP-gluconic acid (lagerstannin B), 3,4-di-O-caffeoylquinic acid and kaempferol-3-(caffeoyldiglucoside)-7-glucoside. method: The chemical used were 1,10-phenathroline, Thibarbituric acid, TRIS-HCl, amominium molybdate, Iron, sodium nitroprusside. HPLC standards were purchased from Sigma-Aldrich. All the reagents were of analytical grade. Double beam spectrophotometer was used for UV-VIS spectrophotometery. The HPLC system used was Agilent 1260 Infinity HPLC system which consists of quaternary pump, degasser, auto-sampler and diode array detector (DAD). conclusion: The results have shown that S. saligna antioxidant rich extract can be effectively utilized in food and pharmaceutical industries with additional income. However, more detailed understanding of utilization of extract against degenerative diseases is required. There is also need to find out the safety and bioavailability of the plant extract for its use as nutraceutical and as a plant products.

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沙棘叶的抗氧化活性和酚类成分

:Sarcocca saligna (D. DON) Muel 是一种重要的植物，含有多种甾体生物碱，对梅毒、肝病、胃肠道疾病、感染和炎症有效。本研究旨在调查 S. saligna 的体外抗氧化活性和酚类概况。高效液相色谱法（HPLC）分析表明，沙参中含有 12 种酚类物质，即山奈酚-3-O-葡萄糖苷、原花青素 B1、槲皮素-3-丙二酰葡萄糖苷-7-葡萄糖苷、山奈酚-3-O-槐糖苷、异鼠李素-3-O-葡萄糖苷-7-Orhamnoside、山奈酚己糖苷、4-O-咖啡酰-5-O-对香豆酰奎宁酸、3,4-二-O-咖啡酰-5-O-阿魏酰奎宁酸、3,4-二-O-阿魏酰-5-O-咖啡酰奎宁酸、黄烷酰-HHDP-葡萄糖酸（拉格斯塔宁 B）、3,4-二-O-咖啡酰奎宁酸和山奈酚-3-（咖啡酰二葡萄糖苷）-7-葡萄糖苷。热水提取物的抗脂质过氧化活性最高。2,2-二苯基-1-苦基肼（DPPH）自由基清除活性的 IC50 值为 71.5 ± 2.1 μg/mL。该提取物能螯合铁，在磷钼试验中具有还原活性。由此得出结论，S. saligna 的提取物含有丰富的抗氧化剂和重要的植物化学物质：本研究旨在调查 S. saligna 的体外抗氧化活性和酚类概况。高效液相色谱分析显示了 12 种酚类物质的存在，即山奈酚-3-O-葡萄糖苷、原花青素 B1、槲皮素-3-丙二酰基葡萄糖苷-7-葡萄糖苷、山奈酚-3-O-槐角苷、异鼠李素-3-O-葡萄糖苷-7-O-鼠李糖苷、山奈酚己糖苷、4-O-咖啡酰-5-O-对香豆酰奎宁酸、3,4-二-O-咖啡酰-5-O-阿魏酰奎宁酸、3,4-二-O-阿魏酰-5-O-咖啡酰奎宁酸、黄加洛尔-HHDP-葡萄糖酸（拉格斯塔宁 B）、3,4-二-O-咖啡酰奎宁酸和山奈酚-3-（咖啡酰二葡萄糖苷）-7-葡萄糖苷。方法：所用化学试剂为 1,10-酚酞、巴比妥酸、TRIS-HCl、钼酸铵、铁、硝普钠。高效液相色谱标准品购自 Sigma-Aldrich。所有试剂均为分析级。紫外-可见分光光度计采用双光束分光光度计。使用的高效液相色谱系统是 Agilent 1260 Infinity 高效液相色谱系统，该系统由四元泵、脱气机、自动进样器和二极管阵列检测器（DAD）组成：研究结果表明，沙参富含抗氧化剂的提取物可有效用于食品和制药行业，并带来额外收入。然而，还需要更详细地了解如何利用萃取物防治退行性疾病。此外，还需要找出植物提取物的安全性和生物利用度，以便将其用作营养保健品和植物产品。

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来源期刊

Letters in Organic Chemistry 化学-有机化学

CiteScore

1.30

自引率

12.50%

发文量

135

审稿时长

7 months

期刊介绍： Aims & Scope Letters in Organic Chemistry publishes original letters (short articles), research articles, mini-reviews and thematic issues based on mini-reviews and short articles, in all areas of organic chemistry including synthesis, bioorganic, medicinal, natural products, organometallic, supramolecular, molecular recognition and physical organic chemistry. The emphasis is to publish quality papers rapidly by taking full advantage of latest technology for both submission and review of the manuscripts. The journal is an essential reading for all organic chemists belonging to both academia and industry.