The Cytotoxic Activity of Phenazine Compounds from Pseudomonas chlororaphis subsp. aurantiaca against the HeLa Cell Line

IF 0.4 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Genetics, Microbiology and Virology Pub Date : 2024-03-20 DOI:10.3103/s0891416823040079

A. A. Zhyzneyskaya, A. A. Lukashevich, N. P. Maksimova, E. G. Veremeenko

引用次数: 0

Abstract

In this investigation, the cytotoxic activity of phenazine compounds from different bacterial strains of Pseudomonas chlororaphis subsp. aurantiaca against the HeLa cervical adenocarcinoma cell line was studied. The cytotoxic concentrations of phenazines against HeLa cells were 300 μg/mL. After incubation with phenazines, cytological preparations of HeLa cells showing the presence of apoptotic bodies were obtained. The effect of phenazines on HeLa cells led to a change in the expression of their ABC transporter genes (abcc1 and abcg2) and tp53. The activity of tp53 increased almost 13-fold, while the expression of the abcc1 and abcg2 genes decreased. The activation of tp53 is one of the probable causes of apoptotic death of HeLa cells in the presence of phenazine compounds from the bacterium P. chlororaphis subsp. aurantiaca.

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绿假单胞杆菌亚种中的吩嗪化合物对 HeLa 细胞系的细胞毒活性

在这项研究中，研究了来自不同绿假丝酵母亚种细菌菌株的酚嗪类化合物对 HeLa 宫颈腺癌细胞系的细胞毒活性。酚嗪类化合物对 HeLa 细胞的细胞毒性浓度为 300 μg/mL。用吩嗪类药物培养后，HeLa 细胞的细胞学制剂显示出凋亡体的存在。酚嗪类化合物对 HeLa 细胞的影响导致其 ABC 转运体基因（abcc1 和 abcg2）和 tp53 的表达发生变化。tp53 的活性几乎增加了 13 倍，而 abcc1 和 abcg2 基因的表达量却下降了。tp53的激活可能是导致HeLa细胞在氯葡萄孢亚种细菌的酚嗪化合物作用下凋亡的原因之一。

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来源期刊

Molecular Genetics, Microbiology and Virology BIOCHEMISTRY & MOLECULAR BIOLOGY-MICROBIOLOGY

CiteScore

0.70

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Molecular Genetics, Microbiology and Virology is a journal that covers most topical theoretical and applied problems of molecular genetics of pro- and eukaryotic organisms, molecular microbiology and molecular virology. An important part the journal assigns to investigations of the genetic apparatus of microorganisms, searching for forms of genetic exchange, genetic mapping of pathogenic causative agents, to ascertainment of the structure and functions of extrachromosomal factors of heredity and migratory genetic elements, to theoretical studies into the mechanisms of genetic regulation. The journal publishes results of research on molecular and genetic bases of an eukaryotic cell, functioning of chromosomes and chromatin, nature of genetic changes in malignization and a set of hereditary diseases. On the pages of the journal there is covered the formulation of molecular bases of virology including issues of integration of viral and cellular genomes, and issues of persistence. The journal plans to put materials on genetic engineering, envisaging synthesis and isolation of genes from natural reservoirs, creation of plasmid- and virus-based vector, production of recombinant DNA molecules, the creation of Gene Banks for Microbes, animals, and human; and also on biotechnological production of hormones, components of antiviral vaccines, diagnostic and therapeutic preparations.