Tina Taikandi, Navid Momenifar, Ali Namvar, Alireza Azizi Saraji, Mehdi Norouzi, Cobra Razavi Pashabayg
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引用次数: 0
Abstract
Human T-lymphotropic virus type 1 (HTLV-1) is associated with the development of malignant diseases, particularly adult T-cell leukemia/lymphoma (ATLL) and myelopathy/tropical spastic paraparesis (HAM/TSP). Hemophilia patients are at risk of acquiring blood–borne infections, making it critical to prevent the transmission of viral and other contaminants. The objective of this study was to determine the prevalence of HTLV-1 in Iranian hemophilia patients who were referred to the Iranian Comprehensive Hemophilia Care Center (ICHCC) and to analyze the results. A total of 320 blood samples were collected from hemophilia patients and screened using enzyme-linked immunosorbent assay (ELISA). Seropositive samples were confirmed by amplifying the long terminal repeat (LTR) region of HTLV-1 using nested PCR. The LTR region fragment was amplified, sequenced, and analyzed by MEGA 7 for phylogenetic analysis. Three out of four positive serological samples were confirmed using PCR, resulting in an HTLV-1 infection outbreak of 0.9%. Phylogenetic analysis of hemophilia patients infected with HTLV-1 revealed that the virus belongs to subtype a (Cosmopolitan) and subgroup A (Transcontinental). The findings suggest that hemophilia patients may be at high risk for HTLV-1 transmission. Furthermore, screening of blood and blood products can play a critical role in preventing the spread of the virus in endemic areas.
期刊介绍：
Molecular Genetics, Microbiology and Virology is a journal that covers most topical theoretical and applied problems of molecular genetics of pro- and eukaryotic organisms, molecular microbiology and molecular virology. An important part the journal assigns to investigations of the genetic apparatus of microorganisms, searching for forms of genetic exchange, genetic mapping of pathogenic causative agents, to ascertainment of the structure and functions of extrachromosomal factors of heredity and migratory genetic elements, to theoretical studies into the mechanisms of genetic regulation. The journal publishes results of research on molecular and genetic bases of an eukaryotic cell, functioning of chromosomes and chromatin, nature of genetic changes in malignization and a set of hereditary diseases. On the pages of the journal there is covered the formulation of molecular bases of virology including issues of integration of viral and cellular genomes, and issues of persistence. The journal plans to put materials on genetic engineering, envisaging synthesis and isolation of genes from natural reservoirs, creation of plasmid- and virus-based vector, production of recombinant DNA molecules, the creation of Gene Banks for Microbes, animals, and human; and also on biotechnological production of hormones, components of antiviral vaccines, diagnostic and therapeutic preparations.
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