RNF20 reduced cell proliferation and Warburg effect of Liver cancer by the promotion of NLRP3 Ubiquitination in liver cancer

Abstract

Background: The present study explored that the effects and its possible mechanisms of ring finger protein 20 (RNF20) in Postoperative survival rate of Liver cancer in clinical. Methods: All the serum samples were collected from our hospital. Quantitative polymerase chain reaction (PCR) and Microarray analysis, and RNA pull down assay were used in this study. Results: We found that the serum RNF20 mRNA expression level in patients with liver cancer were down-regulated. Postoperative survival rate of RNF20 high expression was higher than that of RNF20 low expression. Then, over-expression of RNF20 diminished liver cancer cell proliferation and metastasis. RNF20 reduced Warburg effect of Liver cancer. RNF20 expression regulated NOD-like receptor protein 3 (NLRP3) expression and increased NLRP3 Ubiquitination. NLRP3 participated in the effects of RNF20 on cell proliferation, and not affected on Warburg effect of Liver cancer. Conclusions: Our study demonstrated that the serum RNF20 expression level was down-regulated in Liver cancer, and promoted postoperative survival rate. RNF20 can reduce cancer progression of Liver cancer by NLRP3 signal pathway, suggesting that it may prove to be a potential therapeutic target for postoperative survival rate of Liver cancer.