Inheritance and genetic mapping of the first CPMMV tolerance locus in common bean

IF 2.3 3区农林科学 Q1 AGRONOMY

Plant Pathology Pub Date : 2024-03-16 DOI:10.1111/ppa.13892

Rodrigo S. Silva, Josias C. Faria, Rosana P. Vianello, Paula Arielle M. R. Valdisser, Helton S. Pereira, Leonardo C. Melo, Patrícia V. Pinheiro, Thiago Lívio P. O. Souza

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Abstract

Although cowpea mild mottle virus (CPMMV) has been reported in Brazil since 1983, it has only become a significant concern for researchers and farmers in recent years. The objective of this work was to investigate the genetic basis of CPMMV tolerance, mapping and reporting the first loci associated with this trait in common bean (cv. BRS Sublime). Phenotypic assays were carried out on 180 individual plants (F2 generation) and 180 F2:3 progenies comprising 12 plants per family and their parents (BRS Sublime, tolerant parent × CNFCT 16207, susceptible parent). CPMMV was mechanically inoculated and symptoms were evaluated at 35 days after inoculation, using a 1–5 scoring scale. A linkage map was constructed using 1695 single‐nucleotide polymorphism (SNP) and SilicoDArT markers that segregated in the F2 and F2:3 generations as expected. Markers were distributed across 11 common bean chromosomes, resulting in a total length of 2864 cM, with an average distance between markers of 1.8 cM. Phenotypic observations revealed that tolerance in cv. BRS Sublime is controlled by a single dominant gene. The main effect quantitative trait locus (QTL; CPMMV.Pv08) associated with CPMMV tolerance was identified in the terminal region on chromosome 8 (Pv08). This QTL explained approximately 77% of phenotypic variation, indicating that the inheritance of tolerance to CPMMV is monogenic, controlled by a major locus. As far as we know, this study represents the first investigation into the inheritance and genetic mapping of CPMMV tolerance in common bean, with potential for the development of elite lines with multiple virus resistance/tolerance.

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蚕豆中第一个 CPMMV 耐受基因座的遗传和基因图谱绘制

虽然巴西早在 1983 年就报道了豇豆轻度斑驳病毒（CPMMV），但直到最近几年才引起研究人员和农民的高度关注。这项工作的目的是研究 CPMMV 耐受性的遗传基础，绘制并报告与普通豆类（变种 BRS Sublime）这一性状相关的首个基因位点。对 180 株单株（F2 代）和 180 个 F2:3 后代进行了表型测定，每个家族及其亲本（BRS Sublime，耐受性亲本×CNFCT 16207，易感性亲本）各 12 株。接种 CPMMV 后 35 天，采用 1-5 级评分法对症状进行评估。利用 1695 个单核苷酸多态性（SNP）和 SilicoDArT 标记构建了连接图谱，这些标记在 F2 和 F2:3 代中的分离情况符合预期。标记分布在 11 条普通豆类染色体上，总长度为 2864 cM，标记之间的平均距离为 1.8 cM。表型观察结果表明，BRS Sublime 品种的耐受性是由基因控制的。BRS Sublime 的耐受性由一个显性基因控制。与 CPMMV 耐受性相关的主效应数量性状位点（QTL；CPMMV.Pv08）在第 8 号染色体的末端区域（Pv08）被确定。该 QTL 解释了约 77% 的表型变异，表明对 CPMMV 耐受性的遗传是单基因遗传，由一个主要位点控制。据我们所知，这项研究是对蚕豆 CPMMV 耐受性的遗传和遗传图谱的首次调查，有望培育出具有多种病毒抗性/耐受性的精英品系。

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来源期刊

Plant Pathology 生物-农艺学

CiteScore

5.60

自引率

7.40%

发文量

147

审稿时长

3 months

期刊介绍： This international journal, owned and edited by the British Society for Plant Pathology, covers all aspects of plant pathology and reaches subscribers in 80 countries. Top quality original research papers and critical reviews from around the world cover: diseases of temperate and tropical plants caused by fungi, bacteria, viruses, phytoplasmas and nematodes; physiological, biochemical, molecular, ecological, genetic and economic aspects of plant pathology; disease epidemiology and modelling; disease appraisal and crop loss assessment; and plant disease control and disease-related crop management.