Platelet activation and blood extracellular vesicles: The influence of venepuncture and short blood storage

IF 2.1 4区医学 Q3 HEMATOLOGY

Blood Cells Molecules and Diseases Pub Date : 2024-03-13 DOI:10.1016/j.bcmd.2024.102842

Ivica Marić , Klemen Žiberna , Ana Kolenc , Elvira Maličev

{"title":"Platelet activation and blood extracellular vesicles: The influence of venepuncture and short blood storage","authors":"Ivica Marić , Klemen Žiberna , Ana Kolenc , Elvira Maličev","doi":"10.1016/j.bcmd.2024.102842","DOIUrl":null,"url":null,"abstract":"<div><p>Extracellular vesicles (EVs) as membrane-bound particles released by various cells are potential tools for diagnosis and treatment. Blood cells, particularly platelets, are the source of circulating EVs.</p></div><div><h3>Material</h3><p>EVs were enriched with gradient ultracentrifugation and measured by nanoparticle tracking assay. A flow cytometric multiplex assay was used for cellular source determination. Activation of platelets was measured as a percentage of CD62p+/CD61+ platelets and correlated with the concentration and size of released EVs.</p></div><div><h3>Results</h3><p>In general there was no statistically significant correlation between EVs` concentration and degree of platelet activation. EVs from different cellular sources were detected. Comparing different needle thicknesses, there was a decrease in the EVs concentration for the 16G needle versus the 21G needle, but no difference was observed for EVs` size and phenotype or platelets activation. During blood storage, platelet activation increased, but there was no effect on the EVs` concentration, size, or phenotype.</p></div><div><h3>Conclusions</h3><p>Preanalytical factors like needle thickness and storage time can affect the MVs' properties. Activation of platelets during blood collection or blood storage occurs; however, it is difficult to determine its effect on the physiological properties of EVs since the mechanisms of EVs` biogenesis and especially clearness are not precisely known.</p></div>","PeriodicalId":8972,"journal":{"name":"Blood Cells Molecules and Diseases","volume":"106 ","pages":"Article 102842"},"PeriodicalIF":2.1000,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1079979624000202/pdfft?md5=9311cf50b5aae847a6f9869128d371da&pid=1-s2.0-S1079979624000202-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Blood Cells Molecules and Diseases","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1079979624000202","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"HEMATOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Extracellular vesicles (EVs) as membrane-bound particles released by various cells are potential tools for diagnosis and treatment. Blood cells, particularly platelets, are the source of circulating EVs.

Material

EVs were enriched with gradient ultracentrifugation and measured by nanoparticle tracking assay. A flow cytometric multiplex assay was used for cellular source determination. Activation of platelets was measured as a percentage of CD62p+/CD61+ platelets and correlated with the concentration and size of released EVs.

Results

In general there was no statistically significant correlation between EVs` concentration and degree of platelet activation. EVs from different cellular sources were detected. Comparing different needle thicknesses, there was a decrease in the EVs concentration for the 16G needle versus the 21G needle, but no difference was observed for EVs` size and phenotype or platelets activation. During blood storage, platelet activation increased, but there was no effect on the EVs` concentration, size, or phenotype.

Conclusions

Preanalytical factors like needle thickness and storage time can affect the MVs' properties. Activation of platelets during blood collection or blood storage occurs; however, it is difficult to determine its effect on the physiological properties of EVs since the mechanisms of EVs` biogenesis and especially clearness are not precisely known.

查看原文本刊更多论文

血小板活化与血液细胞外囊泡：静脉穿刺和血液短期储存的影响

细胞外囊泡（EVs）是由各种细胞释放的膜结合颗粒，是诊断和治疗的潜在工具。血细胞，尤其是血小板，是循环EVs的来源。材料用梯度超速离心法富集EVs，并用纳米颗粒追踪测定法进行测量。流式细胞仪多重检测法用于确定细胞来源。血小板的活化以 CD62p+/CD61+ 血小板的百分比来衡量，并与释放的 EVs 的浓度和大小相关。检测到了不同细胞来源的 EVs。比较不同厚度的针头，16G针头与21G针头的EVs浓度有所下降，但EVs的大小、表型或血小板活化程度没有差异。在血液储存期间，血小板活化增加，但对 EVs 的浓度、大小或表型没有影响。血小板在采血或储血过程中会被活化，但很难确定其对 EVs 生理特性的影响，因为 EVs 的生物生成机制，尤其是清晰度还不确切。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Blood Cells Molecules and Diseases 医学-血液学

CiteScore

4.90

自引率

0.00%

发文量

审稿时长

14 days

期刊介绍： Blood Cells, Molecules & Diseases emphasizes not only blood cells, but also covers the molecular basis of hematologic disease and studies of the diseases themselves. This is an invaluable resource to all those interested in the study of hematology, cell biology, immunology, and human genetics.