Functional characterization of sensory neuron membrane protein 1a involved in sex pheromone detection of Apolygus lucorum (Hemiptera: Miridae)

IF 4.6 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY
Yan Li, Xingkui An, Shuang Shan, Xiaoqian Pang, Xiaohe Liu, Yang Sun, Adel Khashaveh, Yong-jun Zhang
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引用次数: 0

Abstract

The mirid bug (Hemiptera: Miridae) is a polyphagous pest that affects a wide range of host plants. Its control remains challenging mainly due to its rapid reproduction, necessitating an understanding of sex pheromone communication. The recognition of sex pheromones is vital for courtship and mating behaviors, and is mediated by various chemosensory-associated proteins. Among these, sensory neuron membrane protein (SNMP), a CD36-related protein, is suggested to play crucial roles in detecting sex pheromones. In this study, we employed transcriptomic and genomic data from . and phylogenetic approaches, and identified four putative genes (, and ) with full open reading frames. Expression analysis revealed the ubiquitous presence of transcripts in multiple tissues, with only exhibiting male-biased expression in the antennae, suggesting its potential role in male chemosensation. Functional analysis using the oocyte expression system, coupled with two-electrode voltage clamp recording, demonstrated that the co-expression of AlucSNMP1a with specific pheromone receptors (PRs) and the co-receptor (Orco) significantly enhanced electrophysiological responses to sex pheromones compared to the co-expression of PRs and Orco alone. Moreover, the results indicated that the presence of AlucSNMP1a not only affected the responsiveness to sex pheromones but also influenced the kinetics (activation and inactivation) of the induced signals. In contrast, the co-expression of AlucSNMP1b with AlucPR/Orco complexes had no impact on the inward currents induced by two pheromone compounds. An examination of the selective pressures on genes across 20 species indicated strong purifying selection, implying potential functional conservation in various insects. These findings highlight the crucial role of AlucSNMP1a in the response to sex pheromones.
参与性信息素检测的感觉神经元膜蛋白 1a 的功能表征
镜蝽(半翅目:镜蝽科)是一种多食性害虫,影响多种寄主植物。由于其繁殖速度快,对其控制仍具有挑战性,因此有必要了解性信息素的传播。性信息素的识别对于求偶和交配行为至关重要,并由各种化学感应相关蛋白介导。其中,感觉神经元膜蛋白(SNMP)是一种与CD36相关的蛋白,被认为在检测性信息素方面起着至关重要的作用。在这项研究中,我们利用.的转录组和基因组数据以及系统发生学方法,确定了四个具有完整开放阅读框的推测基因(、和)。表达分析表明,转录本在多种组织中普遍存在,只有在触角中表现出雄性偏向表达,这表明它在雄性化学感受中的潜在作用。利用卵母细胞表达系统并结合双电极电压钳记录进行的功能分析表明,与单独共表达特定信息素受体(PRs)和共受体(Orco)相比,AlucSNMP1a与特定信息素受体(PRs)和共受体(Orco)的共表达能显著增强对性信息素的电生理反应。此外,研究结果表明,AlucSNMP1a 的存在不仅影响了对性信息素的反应,还影响了诱导信号的动力学(激活和失活)。相反,AlucSNMP1b 与 AlucPR/Orco 复合物共同表达对两种信息素化合物诱导的内向电流没有影响。对 20 个物种的基因选择压力的研究表明,纯化选择作用很强,这意味着各种昆虫的潜在功能保持不变。这些发现凸显了 AlucSNMP1a 在性信息素反应中的关键作用。
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来源期刊
Journal of Integrative Agriculture
Journal of Integrative Agriculture AGRICULTURE, MULTIDISCIPLINARY-
CiteScore
7.90
自引率
4.20%
发文量
4817
审稿时长
3-6 weeks
期刊介绍: Journal of Integrative Agriculture publishes manuscripts in the categories of Commentary, Review, Research Article, Letter and Short Communication, focusing on the core subjects: Crop Genetics & Breeding, Germplasm Resources, Physiology, Biochemistry, Cultivation, Tillage, Plant Protection, Animal Science, Veterinary Science, Soil and Fertilization, Irrigation, Plant Nutrition, Agro-Environment & Ecology, Bio-material and Bio-energy, Food Science, Agricultural Economics and Management, Agricultural Information Science.
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