Genome-wide identification and analysis of expression pattern of REVEILLE transcription factors in castor (Ricinus communis L.)

IF 2.4 3区农林科学 Q1 Agricultural and Biological Sciences

Horticulture Environment and Biotechnology Pub Date : 2024-03-12 DOI:10.1007/s13580-023-00580-5

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引用次数: 0

Abstract

REVEILLE (RVEs) transcription factors play an important role in regulating circadian clock, plant growth and development, and stress responses. While RVEs have been identified in various plant species, comprehensive genome exploration and analysis of RVEs in castor (Ricinus communis L.) have not been previously reported. In this study, we identified RcRVEs in the castor genome through sequence alignment and conducted an analysis encompassing gene structures, protein motifs, physicochemical properties, transcription patterns under cold stress, different tissues, and diurnal rhythm. A total of five RcRVEs were pinpointed in the castor genome. Evolutionary tree analysis categorized these RcRVEs into three distinct groups, implying that different RcRVEs may fulfill distinct biological functions. Furthermore, several abiotic stresses and hormone-responsive cis-acting elements were found in the promoters of the RcRVEs. RNA-sequencing (RNA-seq) analysis revealed that RcRVE1 and RcRVE7 were induced by cold stress and exhibited upregulation over time, indicating their involvement in regulating cold adaptation in castor plants. Five RcRVEs have notably higher expression levels in leaves compared to other tissues. The expression of all RcRVEs displayed diurnal rhythms under light/dark cycles. This study provides valuable insights into the potential functions of RcRVEs in regulating castor’s growth and development, and responses to cold stress.

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蓖麻（Ricinus communis L.）中 REVEILLE 转录因子的全基因组鉴定和表达模式分析

摘要 REVEILLE（RVEs）转录因子在调控昼夜节律、植物生长发育和胁迫反应方面发挥着重要作用。虽然已在多种植物物种中发现了 RVEs，但之前还没有关于蓖麻（Ricinus communis L.）中 RVEs 的全面基因组探索和分析的报道。在本研究中，我们通过序列比对确定了蓖麻基因组中的 RcRVEs，并进行了包括基因结构、蛋白质基序、理化性质、冷胁迫下的转录模式、不同组织和昼夜节律在内的分析。结果在蓖麻基因组中发现了五个RcRVE。进化树分析将这些RcRVE分为三个不同的组，这意味着不同的RcRVE可能具有不同的生物学功能。此外，还在 RcRVEs 的启动子中发现了几种非生物胁迫和激素反应性顺式作用元件。RNA-sequencing（RNA-seq）分析表明，RcRVE1和RcRVE7受到冷胁迫的诱导，并随着时间的推移而上调，表明它们参与调控蓖麻植物的冷适应性。与其他组织相比，五个 RcRVE 在叶片中的表达水平明显较高。在光照/黑暗循环下，所有 RcRVEs 的表达都表现出昼夜节律。这项研究为了解 RcRVEs 在调节蓖麻生长发育和应对冷胁迫方面的潜在功能提供了有价值的见解。

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来源期刊

Horticulture Environment and Biotechnology Agricultural and Biological Sciences-Horticulture

CiteScore

4.30

自引率

4.20%

发文量

审稿时长

6 months

期刊介绍： Horticulture, Environment, and Biotechnology (HEB) is the official journal of the Korean Society for Horticultural Science, was launched in 1965 as the "Journal of Korean Society for Horticultural Science". HEB is an international journal, published in English, bimonthly on the last day of even number months, and indexed in Biosys Preview, SCIE, and CABI. The journal is devoted for the publication of original research papers and review articles related to vegetables, fruits, ornamental and herbal plants, and covers all aspects of physiology, molecular biology, biotechnology, protected cultivation, postharvest technology, and research in plants related to environment.