Maria Luiza S. Mello, Eli H.M. dos Anjos, Benedicto de Campos Vidal
{"title":"Usefulness of sulfonated azo dyes to evaluate macromolecularly oriented protein substrates","authors":"Maria Luiza S. Mello, Eli H.M. dos Anjos, Benedicto de Campos Vidal","doi":"10.1016/j.acthis.2024.152154","DOIUrl":null,"url":null,"abstract":"<div><p>Sulfonated azo dyes are crucial for the histochemical, topochemical, and electrophoretic demonstration of proteins. Additionally, these dyes may reveal the significance of evaluating the anisotropic phenomenon of linear dichroism in macromolecularly oriented stained proteins. However, this requires that the ordered –NH<sub>3</sub><sup>+</sup> groups available for electrostatic binding of the -SO<sub>3</sub><sup>-</sup> dye groups are present in the protein substrate. Further, the reactive -SO<sub>3</sub><sup>-</sup> dye groups should be positioned in a way to permit selective absorption of polarized light at the level of the dye –N<img>N- chromophore azo groups. This review reports the usefulness of sulfonated azo dyes in revealing the extrinsic phenomenon of linear dichroism in dye-substrate complexes and changes in the oriented state of protein macromolecules.</p></div>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0065128124000229/pdfft?md5=89e1a03adb4b8f4ca71b762e43c25173&pid=1-s2.0-S0065128124000229-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0065128124000229","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
引用次数: 0
Abstract
Sulfonated azo dyes are crucial for the histochemical, topochemical, and electrophoretic demonstration of proteins. Additionally, these dyes may reveal the significance of evaluating the anisotropic phenomenon of linear dichroism in macromolecularly oriented stained proteins. However, this requires that the ordered –NH3+ groups available for electrostatic binding of the -SO3- dye groups are present in the protein substrate. Further, the reactive -SO3- dye groups should be positioned in a way to permit selective absorption of polarized light at the level of the dye –NN- chromophore azo groups. This review reports the usefulness of sulfonated azo dyes in revealing the extrinsic phenomenon of linear dichroism in dye-substrate complexes and changes in the oriented state of protein macromolecules.