Usefulness of sulfonated azo dyes to evaluate macromolecularly oriented protein substrates

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Maria Luiza S. Mello, Eli H.M. dos Anjos, Benedicto de Campos Vidal
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引用次数: 0

Abstract

Sulfonated azo dyes are crucial for the histochemical, topochemical, and electrophoretic demonstration of proteins. Additionally, these dyes may reveal the significance of evaluating the anisotropic phenomenon of linear dichroism in macromolecularly oriented stained proteins. However, this requires that the ordered –NH3+ groups available for electrostatic binding of the -SO3- dye groups are present in the protein substrate. Further, the reactive -SO3- dye groups should be positioned in a way to permit selective absorption of polarized light at the level of the dye –NN- chromophore azo groups. This review reports the usefulness of sulfonated azo dyes in revealing the extrinsic phenomenon of linear dichroism in dye-substrate complexes and changes in the oriented state of protein macromolecules.

磺化偶氮染料在评估大分子定向蛋白质底物方面的用途
磺化偶氮染料对蛋白质的组织化学、表观化学和电泳显示至关重要。此外,这些染料还能揭示评估大分子取向染色蛋白质线性二向色的各向异性现象的意义。不过,这需要蛋白质底物中存在有序的 -NH3+ 基团,以便静电结合 -SO3- 染料基团。此外,活性 -SO3- 染料基团的位置应允许染料 -NN- 发色团偶氮基团选择性地吸收偏振光。本综述报告了磺化偶氮染料在揭示染料-底物复合物线性二色性的外在现象以及蛋白质大分子定向状态变化方面的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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