In-depth proteome characterization of endometrium and extraembryonic membranes during implantation in pig.

IF 6.3 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Maria A Gil, Josep M Cambra, Heriberto Rodriguez-Martinez, Cristina Cuello, Inmaculada Parrilla, Emilio A Martinez
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引用次数: 0

Abstract

Background: Proteome characterization of the porcine endometrium and extraembryonic membranes is important to understand mother-embryo cross-communication. In this study, the proteome of the endometrium and chorioallantoic membrane was characterized in pregnant sows (PS) during early gestation (d 18 and 24 of gestation) and in the endometrium of non-pregnant sows (NPS) during the same days using LC-MS/MS analysis. The UniProtKB database and ClueGO were used to obtain functional Gene Ontology annotations and biological and functional networks, respectively.

Results: Our analysis yielded 3,254 and 3,457 proteins identified in the endometrium of PS and NPS, respectively; of these, 1,753 being common while 1,501 and 1,704 were exclusive to PS and NPS, respectively. In addition, we identified 3,968 proteins in the extraembryonic membranes of PS. Further analyses of function revealed some proteins had relevance for the immune system process and biological adhesion in endometrium while the embryonic chorion displayed abundance of proteins related to cell adhesion and cytoskeletal organization, suggesting they dominated the moment of endometrial remodeling, implantation and adhesion of the lining epithelia. Data are available via ProteomeXchange with identifier PXD042565.

Conclusion: This is the first in-depth proteomic characterization of the endometrium and extraembryonic membranes during weeks 3 to 4 of gestation; data that contribute to the molecular understanding of the dynamic environment during this critical period, associated with the majority of pregnancy losses.

猪植入过程中子宫内膜和胚外膜的深度蛋白质组特征。
背景:猪子宫内膜和胚外膜的蛋白质组特征对于了解母体与胚胎的交叉交流非常重要。本研究采用 LC-MS/MS 分析方法,对妊娠早期(妊娠第 18 天和第 24 天)妊娠母猪(PS)和同一天未妊娠母猪(NPS)子宫内膜和绒毛膜的蛋白质组进行了表征。我们利用 UniProtKB 数据库和 ClueGO 分别获得了功能基因本体注释以及生物和功能网络:我们的分析结果表明,在 PS 和 NPS 的子宫内膜中分别发现了 3,254 和 3,457 个蛋白质,其中 1,753 个为常见蛋白质,1,501 和 1,704 个分别为 PS 和 NPS 特有蛋白质。此外,我们还在 PS 胚胎外膜中发现了 3,968 个蛋白质。进一步的功能分析显示,一些蛋白质与免疫系统过程和子宫内膜的生物粘附有关,而胚胎绒毛膜则显示出大量与细胞粘附和细胞骨架组织有关的蛋白质,这表明它们在子宫内膜重塑、植入和内膜上皮粘附的过程中起着主导作用。数据可通过蛋白质组交换(ProteomeXchange)获得,标识符为 PXD042565:这是首次对妊娠第 3 至 4 周的子宫内膜和胚胎外膜进行深入的蛋白质组学描述;这些数据有助于从分子角度了解这一关键时期的动态环境,而大多数妊娠损失都与这一时期有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
10.30
自引率
0.00%
发文量
822
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