Determination of Mycotoxigenic Fungi and Total Aflatoxins in Stored Corn from Sites of Puebla and Tlaxcala, Mexico

Q4 Environmental Science

Nature Environment and Pollution Technology Pub Date : 2024-03-01 DOI:10.46488/nept.2024.v23i01.056

K. Saez-Gomez, R. Avila-Sosa, M. Huerta-Lara, F. Avelino-Flores, R. Munguia-Pérez

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Abstract

This paper aimed to evaluate the contamination with mycotoxigenic fungi and total aflatoxins in stored corn from different sites in Puebla and Tlaxcala, Mexico. Methodology. The study was conducted at two sites in Puebla (San Salvador El Seco and Junta Auxiliar La Resurrección) and two sites in Tlaxcala (Tlaltepango and Nativitas). A total of 80 samples of stored corn were collected. Identification of Aspergillus flavus was performed by microculture techniques and specific taxonomic keys (macromorphological and micromorphological). Then, samples of contaminated corn were selected, and aflatoxin production was confirmed using a direct solid-phase ELISA kit. A total of 25 A. flavus strains were identified. Other possible mycotoxin-producing fungi were Penicillium (n=52) and Fusarium (n=19). Regarding total aflatoxin contamination, all samples were contaminated within a range of 1.589 to 11.854 μg/kg, and the average concentration was 6.3 μg/kg corn. Implications. The detection of mycotoxigenic fungi in the samples tested and of aflatoxins in corn highlights the importance of monitoring these fungi. Since food safety is at risk, it shows the need for methods to control these fungi and their metabolites.

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墨西哥普埃布拉和特拉斯卡拉地区储存玉米中霉菌毒素和黄曲霉毒素总量的测定

本文旨在评估墨西哥普埃布拉州和特拉斯卡拉州不同地点储存的玉米中霉菌毒素真菌和黄曲霉毒素总量的污染情况。研究方法。研究在普埃布拉州的两个地点（San Salvador El Seco 和 Junta Auxiliar La Resurrección）和特拉斯卡拉州的两个地点（Tlaltepango 和 Nativitas）进行。共采集了 80 份储藏玉米样本。黄曲霉的鉴定是通过显微培养技术和特定的分类标准（宏观形态学和微观形态学）进行的。然后，选取受污染的玉米样本，利用直接固相酶联免疫吸附试剂盒确认黄曲霉毒素的产生。共鉴定出 25 株黄曲霉菌株。其他可能产生霉菌毒素的真菌有青霉（52 株）和镰刀菌（19 株）。在黄曲霉毒素总含量方面，所有样本的黄曲霉毒素含量介乎每公斤 1.589 微克至 11.854 微克之间，平均含量为每公斤玉米 6.3 微克。影响。在检测的样本中发现真菌毒素，在玉米中发现黄曲霉毒素，凸显了监测这些真菌的重要性。由于食品安全受到威胁，因此需要采取方法来控制这些真菌及其代谢物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature Environment and Pollution Technology Environmental Science-Environmental Science (all)

CiteScore

1.20

自引率

0.00%

发文量

159

审稿时长

36 weeks

期刊介绍： The journal was established initially by the name of Journal of Environment and Pollution in 1994, whose name was later changed to Nature Environment and Pollution Technology in the year 2002. It has now become an open access online journal from the year 2017 with ISSN: 2395-3454 (Online). The journal was established especially to promote the cause for environment and to cater the need for rapid dissemination of the vast scientific and technological data generated in this field. It is a part of many reputed international indexing and abstracting agencies. The Journal has evoked a highly encouraging response among the researchers, scientists and technocrats. It has a reputed International Editorial Board and publishes peer reviewed papers. The Journal has also been approved by UGC (India). The journal publishes both original research and review papers. The ideology and scope of the Journal includes the following. -Monitoring, control and management of air, water, soil and noise pollution -Solid waste management -Industrial hygiene and occupational health -Biomedical aspects of pollution -Toxicological studies -Radioactive pollution and radiation effects -Wastewater treatment and recycling etc. -Environmental modelling -Biodiversity and conservation -Dynamics and behaviour of chemicals in environment -Natural resources, wildlife, forests and wetlands etc. -Environmental laws and legal aspects -Environmental economics -Any other topic related to environment