Geographic distribution of a missense mutation in the KRT38 gene in Chinese indigenous cattle breeds

IF 1.4 4区农林科学 Q2 Agricultural and Biological Sciences

Animal Production Science Pub Date : 2024-03-07 DOI:10.1071/an22455

Jialei Chen, Xin Liu, Jianyong Liu, Jicai Zhang, Bizhi Huang, Chuzhao Lei

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引用次数: 0

Abstract

Context

China has a vast area across many temperature zones and a variety of cattle breeds. These cattle resources are ideal models to research their adaptability to the environment. The KRT38 gene is an acidic protein, and its coding product can be used as a component of hair production.

Aims

The objective of this study was to investigate the diversity of the KRT38 gene in Chinese local cattle and the association of different genotypes with mean temperature, relative humidity and temperature humidity index.

Methods

A missense mutation g.41650738 A > G in the KRT38 gene was screened from the database of bovine genomic variation (BGVD) and was genotyped in a total of 246 samples from 15 local cattle breeds in China by polymerase chain reaction amplification and sequencing. Finally, the correlation between the locus and the three climatic factors was analysed.

Key results

We successfully obtained the frequency of this single-nucelotide polymorphism in three groups of cattle in northern, central and southern China. The frequency of allele A gradually declined from north to south, whereas the frequency of allele G showed the opposite trend with a clear geographic distribution.

Conclusions

Our results indicate that KRT38 variation in Chinese indigenous cattle might be linked to heat tolerance.

Implications

Our analysis may assist in determining the importance of the variation as a genetic signal for heat tolerance in cattle reproduction and genetics.

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中国本土牛种中 KRT38 基因错义突变的地理分布

背景中国幅员辽阔，跨越多个温度带，牛的品种繁多。这些牛资源是研究其环境适应性的理想模型。KRT38 基因是一种酸性蛋白质，其编码产物可作为毛发生产的成分。目的研究中国地方牛 KRT38 基因的多样性以及不同基因型与平均温度、相对湿度和温湿度指数的关系。方法从牛基因组变异数据库（BGVD）中筛选出 KRT38 基因中的一个错义突变 g.41650738 A > G，并通过聚合酶链式反应扩增和测序对中国 15 个地方牛种共 246 个样本进行基因分型。最后，分析了该位点与三种气候因子之间的相关性。主要结果我们成功获得了该单核苷酸多态性在华北、华中和华南三个牛群中的频率。等位基因 A 的频率由北向南逐渐下降，而等位基因 G 的频率则呈现相反的趋势，且有明显的地理分布。结论我们的研究结果表明，中国土牛的 KRT38 变异可能与耐热性有关。意义我们的分析可能有助于确定该变异作为耐热性遗传信号在牛的繁殖和遗传中的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Animal Production Science Agricultural and Biological Sciences-Food Science

CiteScore

3.00

自引率

7.10%

发文量

139

审稿时长

3-8 weeks

期刊介绍： Research papers in Animal Production Science focus on improving livestock and food production, and on the social and economic issues that influence primary producers. The journal (formerly known as Australian Journal of Experimental Agriculture) is predominantly concerned with domesticated animals (beef cattle, dairy cows, sheep, pigs, goats and poultry); however, contributions on horses and wild animals may be published where relevant. Animal Production Science is published with the endorsement of the Commonwealth Scientific and Industrial Research Organisation (CSIRO) and the Australian Academy of Science.