TAP2 Effect on Min-Pig Stromal Vascular Fraction Cell Gene Expression

Abstract

We have hypothesized that the TAP2 gene is associated with lipid metabolism. Here, 10 Min-pig tissues were collected to detect the expression of TAP2 in different tissues. We obtained dorsal subcutaneous structural vascular fraction (SVF) cells from the Min-pig’s back adipose tissue and induced SVF cells into mature adipocytes. By overexpression and interference, the effect of TAP2 on fat deposition in Min-pig SVF cells was studied. Recombinant human insulin, dexamethasone, indomethacin, 3-isobutyl-1-methylxanthine, triiodothyronine, and rosiglitazone could successfully induce SVF cells into mature adipocytes, and the induction efficiency was above 50%. The tissue expression profile showed that TAP2 was expressed in different tissues, and the highest expression was found in back fat, spleen, and back muscle. Overexpression of the TAP2 gene in SVF cells could significantly increase the expression of adipose differentiation-related genes. The expression of TAP2 in SVF cells was reduced to 0.6 times after transfection of the TAP2 gene interference fragment. The adipogenic transcription factor gene C/EBPα, fatty acid synthase gene FSA, and adipocyte directional differentiation factor ADD1 were downregulated, while the expression of lipolysis-related gene LPL was inhibited. In conclusion, TAP2 expression can promote the deposition of subcutaneous fat on Min-pig’s back adipose tissue.