Effect of 28-Homobrassinolide on Fatty Acid Metabolism During Germination of Crambe tatarica Under Salinity Stress

Pub Date : 2024-03-08 DOI:10.3103/s0095452724010043
S. V. Kretynin, Ya. S. Kolesnikov, V. S. Kravets, Ya. B. Blume
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Abstract

To study the effect of brassinosteroids and salinity stress on the fatty acid metabolism in the seeds of oil plants, the authors analyzed the turnover of fatty acids in the seeds of Crambe tatarica under the mentioned conditions. The results of gas-liquid chromatography and mass-spectrometry demonstrated the decrease in the level of palmitic and linoleic fatty acids along with the increase in the level of oleic, eicosenoic, and docosenoic fatty acids in C. tatarica seeds in response to 28-homobrassinolide under salinity stress on some stages of seed germination. These regularities allowed for the assumption that 28-homobrassinolide promotes the stabilization of fatty acid composition of C. tatarica seeds, disrupted under salinity stress. This may indicate the possibility of modulating the activity or expression of desaturase genes and the enzymes of fatty acid oxidation in the anwer on brassinosteroids.

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28-Homobrassinolide 对盐度胁迫下鞑靼豆发芽过程中脂肪酸代谢的影响
为了研究黄铜类固醇和盐度胁迫对油料植物种子脂肪酸代谢的影响,作者分析了在上述条件下鞑靼文竹(Crambe tatarica)种子中脂肪酸的代谢情况。气液相色谱法和质谱法的结果表明,在种子萌发的某些阶段,鞑靼草种子中的棕榈酸和亚油酸含量降低,而油酸、二十烯酸和二十二烯酸含量增加,这是在盐胁迫下对 28-荷包牡丹碱的反应。根据这些规律,可以推测 28-大黄素内酯促进了鞑靼草种子脂肪酸组成的稳定,而这种稳定在盐胁迫下被破坏了。这可能表明,在黄铜类固醇的作用下,有可能调节去饱和酶基因和脂肪酸氧化酶的活性或表达。
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