Youngkwang Moon, Hui Hun Cho, Hyeokgyun Moon, Hyewon Song, Jae Chul Ro, Jung Heon Lee, Jinkee Lee
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引用次数: 0
Abstract
Lateral flow immunoassay (LFIA) has become a popular method for the rapid detection of biological molecules, with an emerging need for multiplex detection capabilities. A novel LFIA device capable of simultaneously detecting three different antigens on a single test line was developed, with each antigen identifiable by a unique color. Gold nanoparticles (AuNPs; red), gold nanorods (AuNRs; blue), and silver nanoparticles (AgNPs; yellow) were engineered to flow concurrently within the LFIA device and specifically react with α-fetoprotein (AFP), neuron-specific enolase (NSE), and carcinoembryonic antigen (CEA) on the test line. The device was effective for both individual and simultaneous detection of the analytes, with a limit of detection (LOD) of 50 ng/mL. Given its rapid response, ease of use, and affordability, this multiplex detection LFIA device shows great potential for a wide range of applications, including food quality management, livestock diagnosis, and health and environmental monitoring.
期刊介绍：
BioChip Journal publishes original research and reviews in all areas of the biochip technology in the following disciplines, including protein chip, DNA chip, cell chip, lab-on-a-chip, bio-MEMS, biosensor, micro/nano mechanics, microfluidics, high-throughput screening technology, medical science, genomics, proteomics, bioinformatics, medical diagnostics, environmental monitoring and micro/nanotechnology. The Journal is committed to rapid peer review to ensure the publication of highest quality original research and timely news and review articles.
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