Comparative whole genome analysis of face-derived Streptococcus infantis CX-4 unravels the functions related to skin barrier.

IF 1.6 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Genes & genomics Pub Date : 2024-04-01 Epub Date: 2024-03-07 DOI:10.1007/s13258-024-01495-w
Haeun Lee, Dong-Geol Lee, HyungWoo Jo, Young Mok Heo, Chaeyun Baek, Hye-Been Kim, Geunhwa Park, Seunghyun Kang, Wooseok Lee, Seyoung Mun, Kyudong Han
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引用次数: 0

Abstract

Background: The skin microbiome is essential in guarding against harmful pathogens and responding to environmental changes by generating substances useful in the cosmetic and pharmaceutical industries. Among these microorganisms, Streptococcus is a bacterial species identified in various isolation sources. In 2021, a strain of Streptococcus infantis, CX-4, was identified from facial skin and found to be linked to skin structure and elasticity. As the skin-derived strain differs from other S. infantis strains, which are usually of oral origin, it emphasizes the significance of bacterial variation by the environment.

Objective: This study aims to explore the unique characteristics of the CX-4 compared to seven oral-derived Streptococcus strains based on the Whole-Genome Sequencing data, focusing on its potential role in skin health and its possible application in cosmetic strategies.

Methods: The genome of the CX-4 strain was constructed using PacBio Sequencing, with the assembly performed using the SMRT protocol. Comparative whole-genome analysis was then performed with seven closely related strains, utilizing web-based tools like PATRIC, OrthoVenn3, and EggNOG-mapper, for various analyses, including protein association analysis using STRING.

Results: Our analysis unveiled a substantial number of Clusters of Orthologous Groups in diverse functional categories in CX-4, among which sphingosine kinase (SphK) emerged as a unique product, exclusively present in the CX-4 strain. SphK is a critical enzyme in the sphingolipid metabolic pathway, generating sphingosine-1-phosphate. The study also brought potential associations with isoprene formation and retinoic acid synthesis, the latter being a metabolite of vitamin A, renowned for its crucial function in promoting skin cell growth, differentiation, and maintaining of skin barrier integrity. These findings collectively suggest the potential of the CX-4 strain in enhancing of skin barrier functionality.

Conclusion: Our research underscores the potential of the skin-derived S. infantis CX-4 strain by revealing unique bacterial compounds and their potential roles on human skin.

Abstract Image

源自面部的婴儿链球菌 CX-4 的全基因组比较分析揭示了与皮肤屏障有关的功能。
背景:皮肤微生物组在抵御有害病原体和对环境变化做出反应方面至关重要,可产生对化妆品和制药业有用的物质。在这些微生物中,链球菌是在各种分离来源中发现的细菌物种。2021 年,从面部皮肤中鉴定出一株名为 CX-4 的婴儿链球菌,发现它与皮肤结构和弹性有关。由于这种来自皮肤的菌株不同于其他婴儿链球菌菌株,后者通常来自口腔,这就强调了细菌受环境影响而发生变异的重要性:本研究旨在基于全基因组测序数据,探讨 CX-4 与七种口源性链球菌菌株相比的独特特征,重点研究其在皮肤健康中的潜在作用及其在美容策略中的可能应用:方法:使用 PacBio 测序技术构建了 CX-4 菌株的基因组,并使用 SMRT 协议进行了组装。然后利用 PATRIC、OrthoVenn3 和 EggNOG-mapper 等网络工具与 7 个近缘菌株进行了全基因组比较分析,包括利用 STRING 进行蛋白质关联分析:结果:我们的分析揭示了 CX-4 株系中不同功能类别的大量同源群,其中鞘氨醇激酶(SphK)是一个独特的产物,只存在于 CX-4 株系中。SphK 是鞘磷脂代谢途径中的一种关键酶,可生成 1-磷酸鞘磷脂。该研究还发现了异戊二烯形成和视黄酸合成的潜在联系,后者是维生素 A 的代谢产物,在促进皮肤细胞生长、分化和维持皮肤屏障完整性方面具有重要功能。这些发现共同表明,CX-4 菌株具有增强皮肤屏障功能的潜力:我们的研究通过揭示独特的细菌化合物及其对人体皮肤的潜在作用,强调了源于皮肤的婴儿红球菌 CX-4 菌株的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Genes & genomics
Genes & genomics 生物-生化与分子生物学
CiteScore
3.70
自引率
4.80%
发文量
131
审稿时长
6-12 weeks
期刊介绍: Genes & Genomics is an official journal of the Korean Genetics Society (http://kgenetics.or.kr/). Although it is an official publication of the Genetics Society of Korea, membership of the Society is not required for contributors. It is a peer-reviewed international journal publishing print (ISSN 1976-9571) and online version (E-ISSN 2092-9293). It covers all disciplines of genetics and genomics from prokaryotes to eukaryotes from fundamental heredity to molecular aspects. The articles can be reviews, research articles, and short communications.
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