The glycoside hydrolase 7 member VdGH7a regulates Verticillium dahliae pathogenicity and induces host defenses by interacting with GhOLP11

IF 4.6 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY
Junyuan Lv, Shichao Liu, Jinglong Zhou, Zili Feng, Feng Wei, Lihong Zhao, Haiqiang Li, Heqin Zhu, Yalin Zhang, Hongjie Feng
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Abstract

Pathogens secrete multiple enzymes that can degrade the cell wall, thereby weakening the host’s cell wall and facilitating the penetration of the pathogen into the plant. In this study, we identified VdGH7a, a glycoside hydrolase family 7 (GH7) cellobiohydrolase from , which exhibited hydrolytic activity against 1,4-β-glucan. Interestingly, we found that VdGH7a induced cell death in when signal peptides were present. However, this phenomenon was effectively prevented by the carbohydrate-binding type-1 (CBM1) protein domain. Furthermore, we observed that the knockout of VdGH7a significantly reduced the pathogenicity of to cotton plant, as evidenced by the inability of the knockout mutants to penetrate cellophane membrane. Additionally, these knockout mutants displayed diminished ability to exploit carbon sources, rendering them more susceptible to osmotic and cell wall stresses. Moreover, VdGH7a interacted with an osmotin-like protein (GhOLP1) in cotton through yeast two-hybrid screening, and further confirmed using bi-molecular fluorescence complementation (BiFC) and luciferase complementation imaging (LCI). Furthermore, virus-induced gene silencing technology was employed to silence , causing cotton’s salicylic acid (SA) content and resistance to were both reduced, whereas heterologous overexpression of in increased both resistance and the expression of genes involved in the SA signaling pathway. Collectively, these findings demonstrate a virulence strategy whereby the secreted protein VdGH7a from interacts with GhOLP1 to stimulate host immunity and play a significant role in plant resistance against .
糖苷水解酶 7 成员 VdGH7a 通过与 GhOLP11 相互作用调控大丽轮枝菌的致病性并诱导宿主防御能力
病原体会分泌多种能降解细胞壁的酶,从而削弱寄主的细胞壁,促进病原体对植物的渗透。在这项研究中,我们从Ⅳ,Ⅴ,Ⅵ,Ⅶ中鉴定出了一种糖苷水解酶家族 7(GH7)纤维生物水解酶 VdGH7a,它对 1,4-β-葡聚糖具有水解活性。有趣的是,我们发现当信号肽存在时,VdGH7a 会诱导细胞死亡。然而,这种现象被碳水化合物结合型-1(CBM1)蛋白结构域有效地阻止了。此外,我们还观察到,VdGH7a的基因敲除大大降低了对棉花植株的致病性,基因敲除突变体无法穿透玻璃纸膜就是证明。此外,这些基因敲除突变体利用碳源的能力减弱,因此更容易受到渗透胁迫和细胞壁胁迫。此外,通过酵母双杂交筛选,VdGH7a与棉花中的渗透蛋白样蛋白(GhOLP1)发生了相互作用,并通过双分子荧光互补(BiFC)和荧光素酶互补成像(LCI)得到了进一步证实。此外,利用病毒诱导的基因沉默技术沉默了ⅣP1,导致棉花的水杨酸(SA)含量和抗性均降低,而异源过表达ⅣP1则增加了抗性和SA信号通路相关基因的表达。总之,这些发现证明了一种毒力策略,即来自的分泌蛋白 VdGH7a 与 GhOLP1 相互作用,刺激宿主免疫力,并在植物抗穗轴花叶病毒(穗轴花叶病毒)的过程中发挥重要作用。
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来源期刊
Journal of Integrative Agriculture
Journal of Integrative Agriculture AGRICULTURE, MULTIDISCIPLINARY-
CiteScore
7.90
自引率
4.20%
发文量
4817
审稿时长
3-6 weeks
期刊介绍: Journal of Integrative Agriculture publishes manuscripts in the categories of Commentary, Review, Research Article, Letter and Short Communication, focusing on the core subjects: Crop Genetics & Breeding, Germplasm Resources, Physiology, Biochemistry, Cultivation, Tillage, Plant Protection, Animal Science, Veterinary Science, Soil and Fertilization, Irrigation, Plant Nutrition, Agro-Environment & Ecology, Bio-material and Bio-energy, Food Science, Agricultural Economics and Management, Agricultural Information Science.
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