Antibodies from chlamydia-infected individuals facilitate phagocytosis via Fc receptors.

IF 2.9 3区医学 Q3 IMMUNOLOGY

Infection and Immunity Pub Date : 2024-04-09 Epub Date: 2024-03-07 DOI:10.1128/iai.00503-23

Kevin Hybiske, Shahrokh Paktinat, Katherine Newman, Dorothy Patton, Christine Khosropour, Alison C Roxby, Nelly R Mugo, Lynda Oluoch, Kenneth Ngure, Robert Suchland, Florian Hladik, Lucia Vojtech

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引用次数: 0

Abstract

Non-neutralizing functions of antibodies, including phagocytosis, may play a role in Chlamydia trachomatis (CT) infection, but these functions have not been studied and assays are lacking. We utilized a flow-cytometry-based assay to determine whether serum samples from a well-characterized cohort of CT-infected and naïve control individuals enhanced phagocytosis via Fc-receptor-expressing THP-1 cells, and whether this activity correlated with antibody titers. Fc-receptor-mediated phagocytosis was detected only in CT+ donors. Phagocytosis generally did not correlate well with antibody titer. In addition, we found that complement from both CT+ and negative individuals enhanced phagocytosis of CT into primary neutrophils. These results suggest that anti-CT antibodies can have functions that are not reflected by titer. This method could be used to quantitively measure Fc-receptor-mediated function of anti-CT antibodies or complement activity and could reveal new immune correlates of protection.

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衣原体感染者体内的抗体可通过 Fc 受体促进吞噬作用。

抗体的非中和功能，包括吞噬功能，可能在沙眼衣原体（CT）感染中发挥作用，但这些功能尚未得到研究，也缺乏检测方法。我们利用一种基于流式细胞计数法的检测方法来确定来自一组特征明确的 CT 感染者和天真对照者的血清样本是否能通过表达 Fc 受体的 THP-1 细胞增强吞噬作用，以及这种活性是否与抗体滴度相关。仅在 CT+ 供体中检测到 Fc 受体介导的吞噬作用。一般来说，吞噬作用与抗体滴度没有很好的相关性。此外，我们还发现 CT+ 和阴性个体的补体都能增强 CT 对原发性中性粒细胞的吞噬作用。这些结果表明，抗 CT 抗体可能具有滴度无法反映的功能。这种方法可用于定量测量 Fc 受体介导的抗 CT 抗体功能或补体活性，并能揭示新的免疫保护相关因素。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Infection and Immunity 医学-传染病学

CiteScore

6.00

自引率

6.50%

发文量

268

审稿时长

3 months

期刊介绍： Infection and Immunity (IAI) provides new insights into the interactions between bacterial, fungal and parasitic pathogens and their hosts. Specific areas of interest include mechanisms of molecular pathogenesis, virulence factors, cellular microbiology, experimental models of infection, host resistance or susceptibility, and the generation of innate and adaptive immune responses. IAI also welcomes studies of the microbiome relating to host-pathogen interactions.