Measurement of Autophagic Activity in Cancer Cells with Flow Cytometric Analysis Using Cyto-ID Staining.

Q4 Biochemistry, Genetics and Molecular Biology
Merve Şansaçar, Emel Başak Gencer Akçok
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引用次数: 0

Abstract

Autophagy is an evolutionarily conserved process providing the energy that cells need to survive, especially in stress situations, through catabolic processes. Considering the dual role of autophagy in cancer cells depending on the cellular context, it is crucial to comprehend the effect of drug candidates put forward to prevent cancer through the autophagy pathway. The CYTO-ID® Autophagy Detection Kit allows a rapid, specific and quantitative measurement of autophagic activity at the cellular level using a 488 nm-excitable green fluorescent detection reagent via flow cytometer. In this chapter, we present the CYTO-ID® Autophagy Detection method with a stepwise protocol to monitor the autophagy flux after the application of any compound to suspension cancer cell lines with flow cytometric analysis.

利用 Cyto-ID 染色流式细胞分析法测量癌细胞的自噬活性
自噬是一种进化保守过程,通过分解代谢过程为细胞提供生存所需的能量,尤其是在应激情况下。考虑到自噬在癌细胞中的双重作用取决于细胞环境,因此了解候选药物通过自噬途径预防癌症的效果至关重要。CYTO-ID® 自噬检测试剂盒使用 488 纳米可激发绿色荧光的检测试剂,通过流式细胞仪快速、特异、定量地检测细胞水平的自噬活性。在本章中,我们将介绍 CYTO-ID® 自噬检测方法,该方法采用循序渐进的方案,在悬浮癌细胞系中使用任何化合物后,通过流式细胞仪分析监测自噬通量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Methods in molecular biology
Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics
CiteScore
2.00
自引率
0.00%
发文量
3536
期刊介绍: For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.
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