Extracellular vesicles from Fusobacterium nucleatum: roles in the malignant phenotypes of gastric cancer.

IF 3.4 3区 生物学 Q3 CELL BIOLOGY
Cell Cycle Pub Date : 2024-02-01 Epub Date: 2024-03-06 DOI:10.1080/15384101.2024.2324587
Xiangkun Meng, Gang Ma, Xu Zhang, Hua Yin, Yu Miao, Fang He
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引用次数: 0

Abstract

The increase of the Fusobacterium nucleatum level has been previously identified in various cancers including gastric cancer (GC), but how the F. nucleatum exerts its carcinogenic role in GC remains unclear. Several studies revealed that F. nucleatum contributes to cancer progression via its secretion of extracellular vehicles (EVs). Hence, it's designed to reveal the influence of F. nucleatum-derived EVs (Fn-EVs) in GC progression. The tumor and adjacent tissues were collected from 30 GC patients, and the abundance of F. nucleatum was found to be highly expressed in tumor samples. The ultracentrifugation was employed to isolate EVs from F. nucleatum and Escherischia coli (E. coli), which were labeled Fn-EVs and E. coli-EVs, respectively. After treating GC cells with Fn-EVs and E. coli-EVs, cell counting kit 8, colony formation, wound healing as well as transwell assay were performed, which revealed that Fn-EVs effectively enhanced oxaliplatin resistance, and facilitated cell proliferation, migration, invasion, and stemness in GC cells while E. coli-EVs exert no significant effect on GC cells. Besides, the stemness and DNA repair of GC cells were also enhanced by Fn-EVs, as revealed by the sphere-forming assay and the detection of stemness- and DNA repair-associated proteins by western blotting. In vivo analyses demonstrated that Fn-EVs administration not only promoted GC tumor growth and liver metastasis but also conferred GC tumor resistance to oxaliplatin resistance. This study first revealed the contributive role of F. nucleatum in GC development via Fn-EVs, which provided a better perspective for manipulating F. nucleatum in treating GC patients with malignant phenotypes.

核酸镰刀菌的胞外囊泡:在胃癌恶性表型中的作用。
以前曾在包括胃癌(GC)在内的多种癌症中发现过核分枝杆菌水平的升高,但核分枝杆菌如何在胃癌中发挥致癌作用仍不清楚。一些研究显示,核酸酵母菌通过分泌胞外载体(EVs)促进癌症进展。因此,本研究旨在揭示F. nucleatum衍生的EVs(Fn-EVs)对GC进展的影响。研究收集了 30 例 GC 患者的肿瘤和邻近组织,发现 F. nucleatum 在肿瘤样本中高表达。研究人员采用超速离心法从F. nucleatum和Escherischia coli(大肠杆菌)中分离出EVs,分别标记为Fn-EVs和E.用Fn-EVs和大肠杆菌EVs处理GC细胞后,进行了细胞计数试剂盒8、菌落形成、伤口愈合和透孔试验,结果表明Fn-EVs能有效增强奥沙利铂抗性,促进GC细胞的增殖、迁移、侵袭和干性,而大肠杆菌EVs对GC细胞无明显影响。此外,Fn-EVs 还能增强 GC 细胞的干性和 DNA 修复能力,这一点可通过球形成试验和 Western 印迹法检测干性和 DNA 修复相关蛋白得到证实。体内分析表明,服用Fn-EVs不仅能促进GC肿瘤的生长和肝转移,还能使GC肿瘤对奥沙利铂产生耐药性。该研究首次揭示了F. nucleatum通过Fn-EVs在GC发展过程中的促进作用,为操纵F. nucleatum治疗具有恶性表型的GC患者提供了更好的视角。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Cell Cycle
Cell Cycle 生物-细胞生物学
CiteScore
7.70
自引率
2.30%
发文量
281
审稿时长
1 months
期刊介绍: Cell Cycle is a bi-weekly peer-reviewed journal of high priority research from all areas of cell biology. Cell Cycle covers all topics from yeast to man, from DNA to function, from development to aging, from stem cells to cell senescence, from metabolism to cell death, from cancer to neurobiology, from molecular biology to therapeutics. Our goal is fast publication of outstanding research.
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