[Deficiency of cathepsin K improves ischemic angiogenesis in high-fat diet fed mice].

Q3 Medicine
生理学报 Pub Date : 2024-02-25
Xin-Yi Wang, Xu Wang, Zhen-Jie He, Shi-Piao Chen, Wei-Jian Li, Ren-Shan Cui, Hai-Ying Jiang
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引用次数: 0

Abstract

The present study aims to investigate the effect of cathepsin K (CatK) on ischemic angiogenesis in high-fat diet fed mice. The mice were subjected to unilateral hindlimb ischemic surgery, and the ischemic blood flow was measured with a laser Doppler blood flow imager. Immunohistochemical staining was used to observe the quantity of new capillaries in the ischemic lower extremity, and Western blot was used to detect the expression of insulin receptor substrate-1 (IRS-1), p-Akt, Akt and vascular endothelial growth factor (VEGF). Firstly, the effect of high-fat diet on ischemic angiogenesis was observed in wild-type mice, which were randomly divided into control group and high-fat diet group and were fed with normal diet or 60% high-fat diet respectively for 16 weeks. The results showed the body weight and the plasma CatK concentration of the high-fat diet group was significantly increased compared with the control group (P < 0.05), and the blood flow recovery of the high-fat diet group was significantly lower than control group (P < 0.05). Then, wild-type and CatK knock out (CatK-/-) mice were both fed with high-fat diet to further observe the effect and mechanism of CatK on ischemic angiogenesis under high-fat diet. The results showed that the blood flow recovery in the CatK-/- group was significantly greater than the wild-type group, and the number of CD31 positive cells was significantly increased (P < 0.05). At the same time, the protein expression levels of IRS-1, p-Akt and VEGF in the ischemic skeletal muscle were significantly increased in the CatK-/- group compared with the wild-type group (P < 0.05). These results suggest that the deficiency of CatK improves ischemic angiogenesis in high-fat diet fed mice through IRS-1-Akt-VEGF signaling pathway.

[缺乏 cathepsin K 可改善高脂饮食喂养小鼠的缺血性血管生成]。
本研究旨在探讨 cathepsin K(CatK)对高脂饮食喂养小鼠缺血性血管生成的影响。小鼠接受单侧后肢缺血手术,用激光多普勒血流成像仪测量缺血血流量。免疫组化染色观察缺血下肢新生毛细血管的数量,Western blot检测胰岛素受体底物-1(IRS-1)、p-Akt、Akt和血管内皮生长因子(VEGF)的表达。首先,观察高脂饮食对野生型小鼠缺血性血管生成的影响。将野生型小鼠随机分为对照组和高脂饮食组,分别以正常饮食或60%高脂饮食喂养16周。结果显示,与对照组相比,高脂饮食组的体重和血浆CatK浓度明显增加(P<0.05),而高脂饮食组的血流量恢复明显低于对照组(P<0.05)。然后,野生型小鼠和CatK基因敲除(CatK-/-)小鼠均以高脂饮食喂养,进一步观察CatK对高脂饮食下缺血性血管生成的影响和机制。结果显示,CatK-/-组的血流量恢复明显高于野生型组,CD31阳性细胞数量明显增加(P<0.05)。同时,与野生型相比,CatK-/-组缺血骨骼肌中IRS-1、p-Akt和VEGF的蛋白表达水平明显升高(P < 0.05)。这些结果表明,缺乏CatK可通过IRS-1-Akt-VEGF信号通路改善高脂饮食喂养小鼠的缺血性血管生成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
生理学报
生理学报 Medicine-Medicine (all)
CiteScore
1.20
自引率
0.00%
发文量
4820
期刊介绍: Acta Physiologica Sinica (APS) is sponsored by the Chinese Association for Physiological Sciences and Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences (CAS), and is published bimonthly by the Science Press, China. APS publishes original research articles in the field of physiology as well as research contributions from other biomedical disciplines and proceedings of conferences and symposia of physiological sciences. Besides “Original Research Articles”, the journal also provides columns as “Brief Review”, “Rapid Communication”, “Experimental Technique”, and “Letter to the Editor”. Articles are published in either Chinese or English according to authors’ submission.
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