Sources and profiles of toxins in shellfish from the south-central coast of Chile (36°‒ 43° S)

IF 5.5 1区生物学 Q1 MARINE & FRESHWATER BIOLOGY

Harmful Algae Pub Date : 2024-03-01 DOI:10.1016/j.hal.2024.102608

Luis Norambuena-Subiabre , Pamela Carbonell , Pablo Salgado , Claudia Zamora , Oscar Espinoza-González

{"title":"Sources and profiles of toxins in shellfish from the south-central coast of Chile (36°‒ 43° S)","authors":"Luis Norambuena-Subiabre , Pamela Carbonell , Pablo Salgado , Claudia Zamora , Oscar Espinoza-González","doi":"10.1016/j.hal.2024.102608","DOIUrl":null,"url":null,"abstract":"<div>The study of marine toxins in shellfish is of the utmost importance to ensure people's food safety. Marine toxins in shellfish and microalgae in the water column off the south-central coast of Chile (36°‒43° S) were studied in a network of 64 stations over a 14-month period. The relative abundance of harmful species Alexandrium catenella, Alexandrium ostenfeldii, Protoceratium reticulatum, Dinophysis acuminata, Dinophysis acuta, Pseudo-nitzschia seriata group and P. delicatissima group was analyzed. The detection and quantification of lipophilic toxins and domoic acid (DA) in shellfish was determined by UHPLC-MS/MS, and for Paralytic Shellfish Toxins (PSTs) by HPLC-FD with post-column oxidation, while for a culture of A. ostenfeldii a Hylic-UHPLC-MS/MS was used. Results showed that DA, gonyautoxin (GTX)-2, GTX-3 and pectenotoxin (PTX)-2 were detected below the permitted limits, while Gymnodimine (GYM)-A and 13-desmethylespirolide C (SPX-1) were below the limit of quantitation. According to the distribution and abundance record of microalgae, DA would be associated to P. seriata and P. delicatissima-groups, PTX-2 to D. acuminata, and GTX-2, GTX-3, GYM-A, and SPX-1 to A. ostenfeldii. However, the toxin analysis of an A. ostenfeldii culture from the Biobío region only showed the presence of the paralytic toxins C2, GTX-2, GTX-3, GTX-5 and saxitoxin, therefore, the source of production of GYM and SPX is still undetermined.</div>","PeriodicalId":12897,"journal":{"name":"Harmful Algae","volume":"133 ","pages":"Article 102608"},"PeriodicalIF":5.5000,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Harmful Algae","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1568988324000428","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MARINE & FRESHWATER BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

The study of marine toxins in shellfish is of the utmost importance to ensure people's food safety. Marine toxins in shellfish and microalgae in the water column off the south-central coast of Chile (36°‒43° S) were studied in a network of 64 stations over a 14-month period. The relative abundance of harmful species Alexandrium catenella, Alexandrium ostenfeldii, Protoceratium reticulatum, Dinophysis acuminata, Dinophysis acuta, Pseudo-nitzschia seriata group and P. delicatissima group was analyzed. The detection and quantification of lipophilic toxins and domoic acid (DA) in shellfish was determined by UHPLC-MS/MS, and for Paralytic Shellfish Toxins (PSTs) by HPLC-FD with post-column oxidation, while for a culture of A. ostenfeldii a Hylic-UHPLC-MS/MS was used. Results showed that DA, gonyautoxin (GTX)-2, GTX-3 and pectenotoxin (PTX)-2 were detected below the permitted limits, while Gymnodimine (GYM)-A and 13-desmethylespirolide C (SPX-1) were below the limit of quantitation. According to the distribution and abundance record of microalgae, DA would be associated to P. seriata and P. delicatissima-groups, PTX-2 to D. acuminata, and GTX-2, GTX-3, GYM-A, and SPX-1 to A. ostenfeldii. However, the toxin analysis of an A. ostenfeldii culture from the Biobío region only showed the presence of the paralytic toxins C2, GTX-2, GTX-3, GTX-5 and saxitoxin, therefore, the source of production of GYM and SPX is still undetermined.

查看原文本刊更多论文

智利中南部海岸（南纬 36-43 度）贝类毒素的来源和概况

研究贝类中的海洋毒素对确保人们的食品安全至关重要。在为期 14 个月的时间里，对智利中南部海岸（南纬 36°-43°）水体中的贝类和微藻中的海洋毒素进行了研究。分析了有害物种 Alexandrium catenella、Alexandrium ostenfeldii、Protoceratium reticulatum、Dinophysis acuminata、Dinophysis acuta、Pseudo-nitzschia seriata 组和 P. delicatissima 组的相对丰度。采用超高效液相色谱-质谱（UHPLC-MS/MS）对贝类中的亲脂性毒素和多莫酸（DA）进行了检测和定量，采用柱后氧化高效液相色谱-质谱（HPLC-FD）对麻痹性贝类毒素（PSTs）进行了检测和定量，而对 A. ostenfeldii 培养物则采用了 Hylic-UHPLC-MS/MS 方法。结果表明，DA、贡酉毒素（GTX）-2、GTX-3 和果胶毒素（PTX）-2 的检测结果低于允许限值，而 Gymnodimine (GYM)-A 和 13-desmethylespirolide C (SPX-1) 的检测结果低于定量限值。根据微藻的分布和丰度记录，DA 与 P. seriata 和 P. delicatissima 群有关，PTX-2 与 D. acuminata 有关，GTX-2、GTX-3、GYM-A 和 SPX-1 与 A. ostenfeldii 有关。然而，对来自 Biobío 地区的 A. ostenfeldii 培养物进行的毒素分析表明，其中只存在麻痹毒素 C2、GTX-2、GTX-3、GTX-5 和 saxitoxin，因此，GYM 和 SPX 的生产来源仍未确定。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Harmful Algae 生物-海洋与淡水生物学

CiteScore

12.50

自引率

15.20%

发文量

122

审稿时长

7.5 months

期刊介绍： This journal provides a forum to promote knowledge of harmful microalgae and macroalgae, including cyanobacteria, as well as monitoring, management and control of these organisms.