{"title":"Endophytic Bacillus amyloliquefaciens Mdgb15 is a potential biocontrol agent against tree peony gray mold caused by Botrytis cinerea","authors":"","doi":"10.1007/s10658-024-02838-9","DOIUrl":null,"url":null,"abstract":"<h3>Abstract</h3> <p><em>Botrytis cinerea</em> is the causal agent of gray mold disease affecting Paeoniaceae plants. The present study focuses on the potential of <em>Bacillus amyloliquefaciens</em> Mdgb15 as a promising biological control agent toward <em>B. cinerea</em>. The strain Mdgb15 showed the highest inhibition rate (55.56%) among all the strains tested against <em>B. cinerea</em> growth and was effective in controlling tree peony gray mold in detached leaves. A conventional PCR with three specific primer pairs detected lipopetides substance synthesis genes of Mdgb15. The results revealed that Mdgb15 had <em>srfAA</em>, <em>fenA</em> and <em>ituD</em> genes, which resulted in the production of surfactin, fengycin and iturin. An accurate qRT-PCR protocol with specifically designed primers was developed to characterize the expression of lipopeptide synthesis gene of Mdgb15. The qRT-PCR analysis revealed that the expression of the <em>ituD</em> and <em>fenA</em> genes in Mdgb15 was significantly up-regulated during the coculture with <em>B. cinerea</em>. Subsequently, the lipopeptides were extracted by acid precipitation of culture filtrate of Mdgb15 and were separated by Sephadex Gel chromatography. Three separated fractions exhibited strong antifungal activity against <em>B. cinerea</em>, and were identified as bacillomycin D by MALDI-TOF-MS analysis. This study has demonstrated that <em>B. amyloliquefaciens</em> Mdgb15 is a future potential new biocontrol agent for controlling tree peony gray mold disease. It suggests that the antifungal mechanisms of Mdgb15 may be at least partially associated with its ability to secret bacillomycin D.</p>","PeriodicalId":12052,"journal":{"name":"European Journal of Plant Pathology","volume":"29 1","pages":""},"PeriodicalIF":1.7000,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"European Journal of Plant Pathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s10658-024-02838-9","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0
Abstract
Botrytis cinerea is the causal agent of gray mold disease affecting Paeoniaceae plants. The present study focuses on the potential of Bacillus amyloliquefaciens Mdgb15 as a promising biological control agent toward B. cinerea. The strain Mdgb15 showed the highest inhibition rate (55.56%) among all the strains tested against B. cinerea growth and was effective in controlling tree peony gray mold in detached leaves. A conventional PCR with three specific primer pairs detected lipopetides substance synthesis genes of Mdgb15. The results revealed that Mdgb15 had srfAA, fenA and ituD genes, which resulted in the production of surfactin, fengycin and iturin. An accurate qRT-PCR protocol with specifically designed primers was developed to characterize the expression of lipopeptide synthesis gene of Mdgb15. The qRT-PCR analysis revealed that the expression of the ituD and fenA genes in Mdgb15 was significantly up-regulated during the coculture with B. cinerea. Subsequently, the lipopeptides were extracted by acid precipitation of culture filtrate of Mdgb15 and were separated by Sephadex Gel chromatography. Three separated fractions exhibited strong antifungal activity against B. cinerea, and were identified as bacillomycin D by MALDI-TOF-MS analysis. This study has demonstrated that B. amyloliquefaciens Mdgb15 is a future potential new biocontrol agent for controlling tree peony gray mold disease. It suggests that the antifungal mechanisms of Mdgb15 may be at least partially associated with its ability to secret bacillomycin D.
期刊介绍：
The European Journal of Plant Pathology is an international journal publishing original articles in English dealing with fundamental and applied aspects of plant pathology; considering disease in agricultural and horticultural crops, forestry, and in natural plant populations. The types of articles published are :Original Research at the molecular, physiological, whole-plant and population levels; Mini-reviews on topics which are timely and of global rather than national or regional significance; Short Communications for important research findings that can be presented in an abbreviated format; and Letters-to-the-Editor, where these raise issues related to articles previously published in the journal. Submissions relating to disease vector biology and integrated crop protection are welcome. However, routine screenings of plant protection products, varietal trials for disease resistance, and biological control agents are not published in the journal unless framed in the context of strategic approaches to disease management.
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