{"title":"Unraveling the effectiveness of Sargassum wightii against white spot syndrome virus in Paratelphusa hydrodomous: an in-vivo and in-silico approach","authors":"","doi":"10.1007/s12562-024-01758-1","DOIUrl":null,"url":null,"abstract":"<h3>Abstract</h3> <p>White spot syndrome virus (WSSV) is causing significant economic losses in shrimp farming. Envelope proteins, which interact with host cells during viral attachment, are potential targets for drug development. In this study, the antiviral activity of <em>Sargassum wightii</em> was assessed using in-vitro and in-silico analyses. Crabs were injected with <em>S. wightii</em> extract and WSSV and were observed for 30 days post-infection. The antiviral activity was confirmed by bio-assay, histopathology, and in-silico analysis. GC–MS analysis identified 15 compounds in <em>S. wightii</em>, and in-silico docking revealed a strong interaction between VP28 and VP26 envelope proteins and stigmasta-4,22-dien-3-one. Molecular simulations validated the stability of the protein–ligand binding. These findings confirm the potential of <em>S. wightii</em> as a treatment for WSSV.</p>","PeriodicalId":12231,"journal":{"name":"Fisheries Science","volume":"46 1","pages":""},"PeriodicalIF":1.4000,"publicationDate":"2024-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fisheries Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s12562-024-01758-1","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"FISHERIES","Score":null,"Total":0}
引用次数: 0
Abstract
White spot syndrome virus (WSSV) is causing significant economic losses in shrimp farming. Envelope proteins, which interact with host cells during viral attachment, are potential targets for drug development. In this study, the antiviral activity of Sargassum wightii was assessed using in-vitro and in-silico analyses. Crabs were injected with S. wightii extract and WSSV and were observed for 30 days post-infection. The antiviral activity was confirmed by bio-assay, histopathology, and in-silico analysis. GC–MS analysis identified 15 compounds in S. wightii, and in-silico docking revealed a strong interaction between VP28 and VP26 envelope proteins and stigmasta-4,22-dien-3-one. Molecular simulations validated the stability of the protein–ligand binding. These findings confirm the potential of S. wightii as a treatment for WSSV.
期刊介绍：
Fisheries Science is the official journal of the Japanese Society of Fisheries Science, which was established in 1932. Recognized as a leading journal in its field, Fisheries Science is respected internationally for the publication of basic and applied research articles in a broad range of subject areas relevant to fisheries science. All articles are peer-reviewed by at least two experts in the field of the submitted paper. Published six times per year, Fisheries Science includes about 120 articles per volume. It has a rich history of publishing quality papers in fisheries, biology, aquaculture, environment, chemistry and biochemistry, food science and technology, and Social Science.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
