Talal Al-Yazeedi, Sally Adams, Sophie Tandonnet, Anisa Turner, Jun Kim, Junho Lee, Andre Pires-daSilva
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引用次数: 0
Abstract
Auanema freiburgense is a nematode with males, females, and selfing hermaphrodites. When XO males mate with XX females, they typically produce a low proportion of XO offspring because they eliminate nullo-X spermatids. This process ensures that most sperm carry an X chromosome, increasing the likelihood of X chromosome transmission compared to random segregation. This occurs because of an unequal distribution of essential cellular organelles during sperm formation, likely dependent on the X chromosome. Some sperm components are selectively segregated into the X chromosome's daughter cell, while others are discarded with the nullo-X daughter cell. Intriguingly, the interbreeding of 2 A. freiburgense strains results in hybrid males capable of producing viable nullo-X sperm. Consequently, when these hybrid males mate with females, they yield a high percentage of male offspring. To uncover the genetic basis of nullo-spermatid elimination and X chromosome drive, we generated a genome assembly for A. freiburgense and genotyped the intercrossed lines. This analysis identified a quantitative trait locus spanning several X chromosome genes linked to the non-Mendelian inheritance patterns observed in A. freiburgense. This finding provides valuable clues to the underlying factors involved in asymmetric organelle partitioning during male meiotic division and thus non-Mendelian transmission of the X chromosome and sex ratios.
Auanema freiburgense 是一种有雄性、雌性和自交雌雄同体的线虫。当 XO 雄性与 XX 雌性交配时，它们通常会产生较低比例的 XO 后代，因为它们会消除无 X 精子。这一过程可确保大多数精子携带 X 染色体，与随机分离相比，增加了 X 染色体传递的可能性。出现这种情况的原因是精子形成过程中重要细胞器的分布不均，这可能取决于 X 染色体。精子中的某些成分被选择性地分离到 X 染色体的子细胞中，而其他成分则随着无 X 染色体的子细胞被丢弃。耐人寻味的是，两个 A. freiburgense 品系杂交后产生的杂交雄性能产生有活力的无 X 染色体精子。因此，当这些杂交雄性与雌性交配时，它们会产生高比例的雄性后代。为了揭示无精子消除和X染色体驱动的遗传基础，我们生成了弗赖堡蝇的基因组组装，并对杂交品系进行了基因分型。这项分析确定了一个定量性状基因座，该基因座跨越多个 X 染色体基因，与在弗赖堡家蚕中观察到的非孟德尔遗传模式有关。这一发现为了解雄性减数分裂过程中不对称细胞器分配的基本因素，从而了解 X 染色体的非孟德尔遗传和性别比例提供了宝贵的线索。
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GENETICS is published by the Genetics Society of America, a scholarly society that seeks to deepen our understanding of the living world by advancing our understanding of genetics. Since 1916, GENETICS has published high-quality, original research presenting novel findings bearing on genetics and genomics. The journal publishes empirical studies of organisms ranging from microbes to humans, as well as theoretical work.
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