Candidate orexigenic peptide hormone-related genes in yellowtail Seriola quinqueradiata: cloning and tissue distribution of two distinct agouti-related protein genes and response of five candidate appetite-related genes to fasting, fishmeal soluble fraction addition, and a fishmeal-based diet

IF 1.4 4区农林科学 Q3 FISHERIES

Fisheries Science Pub Date : 2024-03-02 DOI:10.1007/s12562-024-01759-0

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引用次数: 0

Abstract

Agouti-related protein (Agrp) is an orexigenic peptide hormone found in fish and other vertebrates. Understanding appetite regulation is important for improving production performance in aquaculture systems. However, information on appetite-related hormones in yellowtail is still fragmentary. In this study, two distinct agrpgenes (i.e., agrp1 and agrp2) were cloned from the yellowtail brain. Brain distribution and response of the agrp genes to fasting, along with other candidate orexigenic peptide hormone-related genes including neuropeptide Y (npy) and melanin-concentrating hormones (mch1 and mch2), were studied. The highest expression level of those genes was observed in the hypothalamus, except for npy. After fasting, agrp1 and npy increased significantly in the hypothalamus, whereas agrp2 in the olfactory bulb and cerebellum decreased significantly. After feeding with a fishmeal (FM)-based diet and addition of a fishmeal soluble fraction (FMS), hypothalamic agrp1, agrp2, mch1, and mch2 increased, whereas hypothalamic npy decreased significantly. From the fasting experiment, agrp1 and npy may function as orexigenic hormones in yellowtail; however, the function of other hormones warrants further research. Expression of all the analyzed appetite-related hormonal genes might respond to the smell/taste of fishmeal through sensory organs. Furthermore, npy and agrp1 may enhance appetite through different mechanisms in yellowtail.

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大黄鱼中与促食欲肽激素相关的候选基因：两个不同的促食欲蛋白相关基因的克隆和组织分布，以及五个与食欲相关的候选基因对禁食、添加鱼粉可溶性成分和鱼粉饮食的反应

摘要 Agouti-related protein（Agrp）是一种存在于鱼类和其他脊椎动物体内的促食欲肽类激素。了解食欲调节对提高水产养殖系统的生产性能非常重要。然而，有关大黄鱼食欲相关激素的信息仍很零散。本研究从大黄鱼脑中克隆了两种不同的 agrp 基因（即 agrp1 和 agrp2）。研究了agrp基因以及其他候选的促矿物质肽类激素相关基因（包括神经肽Y（nyy）和黑色素浓缩激素（mch1和mch2））在大脑中的分布和对禁食的反应。除 npy 外，这些基因在下丘脑的表达水平最高。禁食后，agrp1 和 npy 在下丘脑中的表达量明显增加，而嗅球和小脑中的 agrp2 则明显减少。饲喂以鱼粉（FM）为基础的日粮并添加鱼粉可溶部分（FMS）后，下丘脑中的agrp1、agrp2、mch1和mch2明显增加，而下丘脑中的nyy则明显减少。从空腹实验来看，agrp1和nyy可能是大黄鱼的促食欲激素，但其他激素的功能还有待进一步研究。所有分析的食欲相关激素基因的表达可能通过感觉器官对鱼粉的气味/味道做出反应。此外，npy 和 agrp1 可能通过不同的机制提高大黄鱼的食欲。

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来源期刊

Fisheries Science 农林科学-渔业

CiteScore

3.80

自引率

5.30%

发文量

审稿时长

12-24 weeks

期刊介绍： Fisheries Science is the official journal of the Japanese Society of Fisheries Science, which was established in 1932. Recognized as a leading journal in its field, Fisheries Science is respected internationally for the publication of basic and applied research articles in a broad range of subject areas relevant to fisheries science. All articles are peer-reviewed by at least two experts in the field of the submitted paper. Published six times per year, Fisheries Science includes about 120 articles per volume. It has a rich history of publishing quality papers in fisheries, biology, aquaculture, environment, chemistry and biochemistry, food science and technology, and Social Science.