Response of microbial community diversity and the abundance of nitrogen-cycling genes to Bacillus subtilis application in mulberry field soil

IF 1.2 4区农林科学 Q4 SOIL SCIENCE

Soil Research Pub Date : 2024-02-26 DOI:10.1071/sr23210

Yanfang Yu, Jinzhi Huang, Zhongfei Tong, Zhenhua Deng, Yawei Wang, Junwen Wang

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引用次数: 0

Abstract

Context

Bacillus subtilis (BS) is a widely used microbial agent that could improve soil fertility and soil microenvironment. There is still uncertainty about the suitability of BS for cultivating crops with high demand for nitrogen fertiliser.

Aims

To evaluate the effects of BS agent on microbial community diversity and nitrogen-cycling genes in mulberry rhizosphere soil.

Methods

Pot experiments were conducted. Different dosages (CK, 0; T1, 0.5 × 10⁶ CFU g⁻¹ soil; T2, 1 × 10⁶ CFU g⁻¹ soil; T3, 2 × 10⁶ CFU g⁻¹ soil) of BS agent were applied to irrigate the mulberry soil. The soil nutrient content, enzyme activity, bacterial community, and nitrogen-cycling genes were determined.

Key results

T1 had the highest Chao1 and Shannon index, while T3 had the lowest. BS-treated samples had higher relative abundance of Actinobacteria and Chloroflexi than that of CK. Specially, BS-treated samples had higher relative abundance of Sphingomonas, Reyranella, and Hyphomicrobium, which was significantly positively correlated with the content of organic matter, total soluble nitrogen, ammonium nitrogen, and the activity of sucrase. The abundance of genes involved in amino acid metabolism, energy metabolism, metabolism of cofactors, and vitamin functions also increased in the BS-treated samples. BS treatment significantly increased the abundance of AOA-amoA and nirK genes, but decreased the abundance of nirS and nifH genes.

Conclusions

An appropriate amount of BS agent could improve soil fertility, regulate the dominant bacterium communities, and affect the abundance of functional genes involved in nitrogen cycling.

Implications

BS is probably a good choice for mulberry cultivation to improve nitrogen fertiliser utilisation efficiency.

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桑田土壤中微生物群落多样性和氮循环基因丰度对枯草芽孢杆菌应用的响应

背景枯草芽孢杆菌（BS）是一种广泛使用的微生物制剂，可改善土壤肥力和土壤微环境。目前尚不确定枯草芽孢杆菌是否适用于种植氮肥需求量大的作物。目的评估 BS 药剂对桑树根圈土壤微生物群落多样性和氮循环基因的影响。方法进行盆栽实验。施用不同剂量的 BS 剂（CK，0；T1，0.5 × 106 CFU g-1 土壤；T2，1 × 106 CFU g-1 土壤；T3，2 × 106 CFU g-1 土壤）灌溉桑园土壤。测定了土壤养分含量、酶活性、细菌群落和氮循环基因。主要结果T1 的 Chao1 和香农指数最高，而 T3 最低。经 BS 处理的样品中放线菌和绿僵菌的相对丰度高于 CK 样品。特别是，BS 处理过的样品中鞘氨单胞菌、雷氏菌和拟杆菌的相对丰度较高，与有机质含量、可溶性总氮、铵态氮和蔗糖酶活性呈显著正相关。参与氨基酸代谢、能量代谢、辅助因子代谢和维生素功能的基因丰度在 BS 处理的样品中也有所增加。BS 处理明显增加了 AOA-amoA 和 nirK 基因的丰度，但降低了 nirS 和 nifH 基因的丰度。结论适量的 BS 药剂可以提高土壤肥力，调节优势菌群落，影响氮循环功能基因的丰度。ImplicationsBS可能是桑树栽培提高氮肥利用效率的一个不错的选择。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Soil Research SOIL SCIENCE-

CiteScore

3.20

自引率

6.20%

发文量

审稿时长

4.5 months

期刊介绍： Soil Research (formerly known as Australian Journal of Soil Research) is an international journal that aims to rapidly publish high-quality, novel research about fundamental and applied aspects of soil science. As well as publishing in traditional aspects of soil biology, soil physics and soil chemistry across terrestrial ecosystems, the journal welcomes manuscripts dealing with wider interactions of soils with the environment. Soil Research is published with the endorsement of the Commonwealth Scientific and Industrial Research Organisation (CSIRO) and the Australian Academy of Science.