Two distinct subpopulations of marginal zone B cells exhibit differential antibody-producing capacities and radioresistance

IF 21.8 1区 医学 Q1 IMMUNOLOGY
Sujin Lee, Yeunjung Ko, Hyun Woo Lee, Won Joon Oh, Hun Gi Hong, Dinuka Ariyaratne, Se Jin Im, Tae Jin Kim
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Abstract

Marginal zone (MZ) B cells, which are splenic innate-like B cells that rapidly secrete antibodies (Abs) against blood-borne pathogens, are composed of heterogeneous subpopulations. Here, we showed that MZ B cells can be divided into two distinct subpopulations according to their CD80 expression levels. CD80high MZ B cells exhibited greater Ab-producing, proliferative, and IL-10-secreting capacities than did CD80low MZ B cells. Notably, CD80high MZ B cells survived 2-Gy whole-body irradiation, whereas CD80low MZ B cells were depleted by irradiation and then repleted with one month after irradiation. Depletion of CD80low MZ B cells led to accelerated development of type II collagen (CII)-induced arthritis upon immunization with bovine CII. CD80high MZ B cells exhibited higher expression of genes involved in proliferation, plasma cell differentiation, and the antioxidant response. CD80high MZ B cells expressed more autoreactive B cell receptors (BCRs) that recognized double-stranded DNA or CII, expressed more immunoglobulin heavy chain sequences with shorter complementarity-determining region 3 sequences, and included more clonotypes with no N-nucleotides or with B-1a BCR sequences than CD80low MZ B cells. Adoptive transfer experiments showed that CD21+CD23+ transitional 2 MZ precursors preferentially generated CD80low MZ B cells and that a proportion of CD80low MZ B cells were converted into CD80high MZ B cells; in contrast, CD80high MZ B cells stably remained CD80high MZ B cells. In summary, MZ B cells can be divided into two subpopulations according to their CD80 expression levels, Ab-producing capacity, radioresistance, and autoreactivity, and these findings may suggest a hierarchical composition of MZ B cells with differential stability and BCR specificity.

Abstract Image

Abstract Image

边缘区 B 细胞的两个不同亚群表现出不同的抗体生成能力和放射抗性。
边缘区(MZ)B细胞是脾脏先天性B细胞,能快速分泌抗体(Abs)对抗血源性病原体,它由不同的亚群组成。在这里,我们发现 MZ B 细胞可根据其 CD80 表达水平分为两个不同的亚群。与 CD80 低的 MZ B 细胞相比,CD80 高的 MZ B 细胞具有更强的抗体生成、增殖和 IL-10 分泌能力。值得注意的是,CD80高的MZ B细胞能在2-Gy全身辐照后存活,而CD80低的MZ B细胞则会因辐照而耗竭,并在辐照一个月后重新耗竭。CD80低MZ B细胞的耗竭导致牛CII免疫后II型胶原蛋白(CII)诱导的关节炎加速发展。CD80 高的 MZ B 细胞在涉及增殖、浆细胞分化和抗氧化反应的基因中表现出更高的表达量。与 CD80 低的 MZ B 细胞相比,CD80 高的 MZ B 细胞表达更多识别双链 DNA 或 CII 的自反应性 B 细胞受体(BCR),表达更多具有较短互补决定区 3 序列的免疫球蛋白重链序列,并包含更多无 N 核苷酸或具有 B-1a BCR 序列的克隆型。采用性转移实验表明,CD21+CD23+过渡性2 MZ前体优先生成CD80低MZ B细胞,一部分CD80低MZ B细胞转化为CD80高MZ B细胞;相比之下,CD80高MZ B细胞稳定地保持为CD80高MZ B细胞。总之,MZ B细胞可根据其CD80表达水平、Ab生成能力、放射抗性和自反应性分为两个亚群,这些发现可能表明MZ B细胞的组成是分层的,具有不同的稳定性和BCR特异性。
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来源期刊
CiteScore
31.20
自引率
1.20%
发文量
903
审稿时长
1 months
期刊介绍: Cellular & Molecular Immunology, a monthly journal from the Chinese Society of Immunology and the University of Science and Technology of China, serves as a comprehensive platform covering both basic immunology research and clinical applications. The journal publishes a variety of article types, including Articles, Review Articles, Mini Reviews, and Short Communications, focusing on diverse aspects of cellular and molecular immunology.
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