AMELX gene association to early childhood caries in south-Indian children: a case-control study.

IF 2.3 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

European Archives of Paediatric Dentistry Pub Date : 2024-04-01 Epub Date: 2024-02-26 DOI:10.1007/s40368-024-00866-x

A Sharma, M S Muthu, V Vettriselvi, S Nuvvula, T Gayathri

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引用次数: 0

Abstract

Purpose: Genetic variants of AMELX gene can affect the protein content, organization of enamel prisms, microstructure and microhardness of the enamel, thus altering the caries susceptibility. The present study aims to assess the association between polymorphisms rs17878486, rs5934997, and rs5933871 of AMELX gene and Early Childhood Caries (ECC).

Materials and methods: This case-control study was conducted on 200 participants, aged 3-6 years, with 100 controls and 100 children with ECC. A questionnaire was used to collect demographic data, birth-weight, type of delivery, oral hygiene practices, feeding history and 24-h diet diary. DNA was isolated from blood and subjected to PCR followed by Sanger sequencing.

Results: The CC genotype of rs17878486 showed an OR of 1.93 (0.34-10.81; P = 0.73). In a recessive model, the CC genotype of rs17878486 reported an OR of 2.04 (0.36-11.40; P = 0.68); rs5593871 reported an OR of 1.00 (0.31-3.21). Statistically significant differences (P ≤ 0.05) between genotype and allele frequencies of rs17878486, rs5934997, and rs5933871 were not observed between children with ECC and the controls.

Conclusion: Polymorphisms of AMELX gene did not show a significant association with ECC in this population. However, documentation of genetic data in a global context of ECC may be essential for the future.

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AMELX 基因与南印度儿童早期龋齿的关系：一项病例对照研究。

目的：AMELX基因的遗传变异可影响釉质的蛋白质含量、釉棱组织、微结构和微硬度，从而改变龋病的易感性。本研究旨在评估 AMELX 基因多态性 rs17878486、rs5934997 和 rs5933871 与儿童早期龋齿（ECC）之间的关联：这项病例对照研究以 200 名 3-6 岁的参与者为对象，其中包括 100 名对照组和 100 名 ECC 患儿。调查问卷用于收集人口统计学数据、出生体重、分娩类型、口腔卫生习惯、喂养史和 24 小时饮食日记。从血液中分离出DNA，然后进行PCR和Sanger测序：rs17878486的CC基因型的OR值为1.93（0.34-10.81；P = 0.73）。在隐性模型中，rs178486的CC基因型的OR值为2.04（0.36-11.40；P = 0.68）；rs5593871的OR值为1.00（0.31-3.21）。rs17878486、rs5934997和rs5933871的基因型和等位基因频率在ECC患儿和对照组之间没有统计学意义上的差异（P≤0.05）：结论：在这一人群中，AMELX 基因的多态性与 ECC 没有明显关联。结论：在这一人群中，AMELX 基因的多态性并未显示出与 ECC 的显著关联。然而，在未来，记录 ECC 全球背景下的遗传数据可能是至关重要的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

European Archives of Paediatric Dentistry DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

4.40

自引率

9.10%

发文量

期刊介绍： The aim and scope of European Archives of Paediatric Dentistry (EAPD) is to promote research in all aspects of dentistry for children, including interceptive orthodontics and studies on children and young adults with special needs. The EAPD focuses on the publication and critical evaluation of clinical and basic science research related to children. The EAPD will consider clinical case series reports, followed by the relevant literature review, only where there are new and important findings of interest to Paediatric Dentistry and where details of techniques or treatment carried out and the success of such approaches are given.