The Impact of Follicular Fluid on the in vitro Maturation of Camel Oocytes

Abstract

Background: The objective of this research is to find out how much of an impact adding camel follicular fluid (CFF) to camel oocytes in vitro maturation (IVM) medium has on getting meiosis back on track. Methods: Ovaries from camels were obtained at the central slaughterhouse in Riyadh, Kingdom of Saudi Arabia (KSA). Oocytes were extracted from follicles ranging in size from 2 to 8 mm. Camel oocytes were cultivated in a maturation medium containing varying amounts of camel follicular fluid: 0% (control), 2.5% and 5% for a duration of 32-36 hours. Result: According to the result, the maturation rate of oocytes was considerably (p.05) lower in the 2.5% CFF and 5% CFF groups compared to the control group. It is advised that greater CFF concentrations of camel oocytes be added to the IVM culture in order to improve nuclear and cytoplasmic maturation and boost oocyte competency.