The bacterial community of the European spruce bark beetle in space and time

IF 5.6 1区农林科学 Q1 ENTOMOLOGY

Entomologia Generalis Pub Date : 2024-02-23 DOI:10.1127/entomologia/2023/2114

Abdelhameed Moussa, Stefano Nones, Patrizia Elena Vannucchi, Gul-i-Rayna Shahzad, Jessica Dittmer, Erika Corretto, Martin Schebeck, Massimo Faccoli, Andrea Battisti, Christian Stauffer, Hannes Schuler

{"title":"The bacterial community of the European spruce bark beetle in space and time","authors":"Abdelhameed Moussa, Stefano Nones, Patrizia Elena Vannucchi, Gul-i-Rayna Shahzad, Jessica Dittmer, Erika Corretto, Martin Schebeck, Massimo Faccoli, Andrea Battisti, Christian Stauffer, Hannes Schuler","doi":"10.1127/entomologia/2023/2114","DOIUrl":null,"url":null,"abstract":"The European spruce bark beetle Ips typographus is a pest causing severe damage in forests dominated by Norway spruce in Europe. Microorganisms play essential roles in bark beetles, including nutrition, fitness as well as in overcoming host defenses. Here, we performed high-throughput 16S rRNA metabarcoding of I. typographus across different populations in Europe, to assess its bacterial community. We investigated four postglacial refugial areas in Europe and focused specifically on a current bark beetle hot spot in the Dolomites where we compared populations with different epidemiological phases (outbreaking vs. non-outbreaking) and across different seasons (pre-overwintering vs. overwintering). Our results show that the bacterial community structure varied among populations from the refugial areas and between different regions within the Dolomites. We found a significant difference in the bacterial community between pre-overwintering and overwintering individuals, but we did not find differences between epidemic and endemic populations. The prevalence of the genera Erwinia (which was present in all individuals) and Pseudoxanthomonas (present in almost all individuals) across all populations suggests that these taxa form the core bacterial community of I. typographus. Furthermore, several additional bacterial taxa occurred in all populations, but with variable frequencies. This study highlights a complex interaction of I. typographus and various bacterial taxa across different regions and ecological phases and provides new insights into the role of microorganisms in the biology of this important pest species.","PeriodicalId":11728,"journal":{"name":"Entomologia Generalis","volume":"41 1","pages":""},"PeriodicalIF":5.6000,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Entomologia Generalis","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1127/entomologia/2023/2114","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENTOMOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

The European spruce bark beetle Ips typographus is a pest causing severe damage in forests dominated by Norway spruce in Europe. Microorganisms play essential roles in bark beetles, including nutrition, fitness as well as in overcoming host defenses. Here, we performed high-throughput 16S rRNA metabarcoding of I. typographus across different populations in Europe, to assess its bacterial community. We investigated four postglacial refugial areas in Europe and focused specifically on a current bark beetle hot spot in the Dolomites where we compared populations with different epidemiological phases (outbreaking vs. non-outbreaking) and across different seasons (pre-overwintering vs. overwintering). Our results show that the bacterial community structure varied among populations from the refugial areas and between different regions within the Dolomites. We found a significant difference in the bacterial community between pre-overwintering and overwintering individuals, but we did not find differences between epidemic and endemic populations. The prevalence of the genera Erwinia (which was present in all individuals) and Pseudoxanthomonas (present in almost all individuals) across all populations suggests that these taxa form the core bacterial community of I. typographus. Furthermore, several additional bacterial taxa occurred in all populations, but with variable frequencies. This study highlights a complex interaction of I. typographus and various bacterial taxa across different regions and ecological phases and provides new insights into the role of microorganisms in the biology of this important pest species.

查看原文本刊更多论文

欧洲云杉树皮甲虫细菌群落的时空变化

欧洲云杉树皮甲虫 Ips typographus 是一种害虫，对欧洲以挪威云杉为主的森林造成严重破坏。微生物在树皮甲虫体内发挥着至关重要的作用，包括营养、适应性以及克服宿主防御。在这里，我们对欧洲不同种群中的I. typographus进行了高通量16S rRNA代谢编码，以评估其细菌群落。我们调查了欧洲的四个冰川后避难区，并特别关注了多洛米蒂目前的一个树皮甲虫热点地区，比较了不同流行病学阶段（爆发期与非爆发期）和不同季节（越冬前与越冬期）的种群。我们的研究结果表明，细菌群落结构在来自避难区的种群之间以及多洛米蒂山脉不同地区之间存在差异。我们发现越冬前个体和越冬个体之间的细菌群落存在明显差异，但我们没有发现流行病种群和地方病种群之间存在差异。Erwinia 属（存在于所有个体中）和假黄单胞菌属（几乎存在于所有个体中）在所有种群中都很普遍，这表明这些类群构成了 typographus 的核心细菌群落。此外，其他一些细菌类群也出现在所有种群中，但出现频率各不相同。这项研究强调了I. typographus与不同细菌类群在不同地区和生态阶段的复杂相互作用，并为了解微生物在这一重要害虫物种生物学中的作用提供了新的视角。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Entomologia Generalis 生物-昆虫学

CiteScore

7.10

自引率

18.80%

发文量

审稿时长

>12 weeks

期刊介绍： Its scope covers all aspects of basic and applied research dealing with insects and more broadly with arthropods inhabiting wild, agricultural and/or urban habitats. The journal also considers research integrating various disciplines and issues within the broad field of entomology and ecology. Entomologia Generalis publishes high quality research articles on advances in knowledge on the ecology and biology of arthropods, as well as on their importance for key ecosystems services, e.g. as biological control and pollination. The journal devotes special attention to contributions providing significant advances (i) on the fundamental knowledge and on sustainable control strategies of arthropod pests (including of stored products) and vectors of diseases, (ii) on the biology and ecology of beneficial arthropods, (iii) on the spread and impact of invasive pests, and (iv) on potential side effects of pest management methods. Entomologia Generalis welcomes review articles on significant developments in the field of entomology. These are usually invited by the editorial board, but proposals may be sent to the Editor-in-Chief for preliminary assessment by the editorial board before formal submission to the journal. The journal also considers comments on papers published in Entomologia Generalis, as well as short notes on topics that are of broader interest.