Isolation and antioxidant activity of peptides from Chinese hairy tofu

IF 1.8 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Peptide Science Pub Date : 2024-02-23 DOI:10.1002/psc.3572

Li-Ping Wu, Yong-Xiang Wu, Xiang-Tao Ke, Pan Wang, Shuo Zhang, Yu-Ting Zhu, Ying Lu, Yu-Jie Shu, Shang-Yue Jiang, Chang-Jiang Li, Xiao-Qian Hu

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Abstract

Hairy tofu is a famous Chinese snack that is made from soybeans and rich in various nutrients. In order to further explore the antioxidant peptides of hairy tofu hydrolysates, seven proteases were used to hydrolyze hairy tofu. The results of in vitro radical scavenging activity showed that hairy tofu hydrolysates obtained by pancreatin exhibited the highest antioxidant activity. After Sephadex G-25 gel filtration and reversed-phase high-performance liquid chromatography (RP-HPLC), 97 peptides were identified in the most antioxidant fraction using liquid chromatography-mass spectrometry/mass spectrometry (LC–MS/MS). Among them, nine peptides were synthesized and their antioxidant activities were assessed using a H₂O₂-induced oxidative 293T cell model. Finally, four peptides (QCESHK, LAWNEGR, NLQGENEWDQK, and FTEMWR) at concentrations of < 50 μg/ml significantly decreased the malondialdehyde content compared with the model group, displaying in vivo antioxidant activity and low cytotoxicity. Overall, this research provided the choice of using hairy tofu peptides as antioxidant products in the pharmaceutical and food industries.

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从中国毛豆腐中分离多肽并提高其抗氧化活性

毛豆腐是中国著名的小吃，由大豆制成，富含多种营养物质。为了进一步探究毛豆腐水解物的抗氧化肽，研究人员使用了七种蛋白酶水解毛豆腐。体外自由基清除活性结果表明，胰蛋白酶水解的毛豆腐具有最高的抗氧化活性。经过Sephadex G-25凝胶过滤和反相高效液相色谱法（RP-HPLC），液相色谱-质谱/质谱联用（LC-MS/MS）在抗氧化性最强的部分中鉴定出97个肽段。合成了其中的九种肽，并利用 H2 O2 诱导的 293T 细胞氧化模型评估了它们的抗氧化活性。最后，与模型组相比，浓度小于 50 μg/ml 的四种多肽（QCESHK、LAWNEGR、NLQGENEWDQK 和 FTEMWR）显著降低了丙二醛含量，显示出体内抗氧化活性和低细胞毒性。总之，这项研究为将毛豆腐肽作为抗氧化产品用于制药和食品行业提供了选择。

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来源期刊

Journal of Peptide Science 生物-分析化学

CiteScore

3.40

自引率

4.80%

发文量

审稿时长

1.7 months

期刊介绍： The official Journal of the European Peptide Society EPS The Journal of Peptide Science is a cooperative venture of John Wiley & Sons, Ltd and the European Peptide Society, undertaken for the advancement of international peptide science by the publication of original research results and reviews. The Journal of Peptide Science publishes three types of articles: Research Articles, Rapid Communications and Reviews. The scope of the Journal embraces the whole range of peptide chemistry and biology: the isolation, characterisation, synthesis properties (chemical, physical, conformational, pharmacological, endocrine and immunological) and applications of natural peptides; studies of their analogues, including peptidomimetics; peptide antibiotics and other peptide-derived complex natural products; peptide and peptide-related drug design and development; peptide materials and nanomaterials science; combinatorial peptide research; the chemical synthesis of proteins; and methodological advances in all these areas. The spectrum of interests is well illustrated by the published proceedings of the regular international Symposia of the European, American, Japanese, Australian, Chinese and Indian Peptide Societies.