Factorial design-assisted spectrophotometric methods for the determination of total catechins in green tea extract via reaction with MBTH. Application to commercial tablet

Monatshefte für Chemie / Chemical Monthly Pub Date : 2024-02-21 DOI:10.1007/s00706-024-03177-5

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Abstract

Green tea extract (GTE) is a supplement derived from the green tea plant which aids in weight loss along with other innumerable health. New analytical methods for determining GTE via its catechins using a design-assisted spectrophotometric methodologies are provided. The procedures are based on the formation of orange-colored products that are detectable at 473 or 455 nm, respectively, when catechins react with MBTH in the presence of FeCl₃ (method I) or K₂S₂O₈ (method II). Critical parameters were optimized using design of experiments (DOE). The absorbance-concentration plots were rectilinear over the ranges 4.0–20.0 or 4.0–40.0 µg cm⁻³ with limits of detection 0.393 or 0.493 µg cm⁻³ for methods I & II, respectively. Our methods were applied to pure catechins in GTE, namely (-)-epicatechin and (-)-epigallocatechin gallate. Moreover, the proposed methods were perfectly applied to the analysis of GTE in a commercial tablet. Good agreement between the collected data and those of the reported approach demonstrating that there were no significant variations. According to International Council for Harmonization (ICH) Guidelines, the procedures were validated.

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通过与 MBTH 反应测定绿茶提取物中总儿茶素的因子设计辅助分光光度法。应用于商业片剂

摘要绿茶提取物（GTE）是从绿茶植物中提取的一种补充剂，有助于减肥和其他多种健康。本文提供了利用设计辅助分光光度法通过儿茶素测定 GTE 的新分析方法。这些方法基于儿茶素与 MBTH 在 FeCl3（方法 I）或 K2S2O8（方法 II）存在下发生反应时形成的橙色产物，这些产物分别可在 473 或 455 纳米波长下检测到。利用实验设计（DOE）对关键参数进行了优化。吸光度-浓度图在 4.0-20.0 或 4.0-40.0 µg cm-3 范围内呈直线，方法 I & II 的检出限分别为 0.393 或 0.493 µg cm-3。我们的方法适用于 GTE 中的纯儿茶素，即(-)-表儿茶素和(-)-表没食子儿茶素没食子酸酯。此外，所提出的方法还完美地应用于商品片剂中儿茶素的分析。收集到的数据与报告方法的数据非常吻合，表明没有明显的差异。根据国际协调理事会（ICH）指南，该方法通过了验证。图表摘要

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Monatshefte für Chemie / Chemical Monthly

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