Transcriptome Dynamics and Cell Dialogs Between Oocytes and Granulosa Cells in Mouse Follicle Development

IF 11.5 2区 生物学 Q1 GENETICS & HEREDITY
Wenju Liu, Xinyu Cui, Yuhan Zhang, Liang Gu, Yuanlin He, Jing Li, Shaorong Gao, Rui Gao, Cizhong Jiang
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Abstract The development and maturation of follicles is a sophisticated and multistage process. The dynamic gene expression of oocytes and the surrounding somatic cells and the dialogs between these cells are critical to this process. We accurately classified the follicle development into nine stages and profiled the gene expression of mouse oocytes, the companion granulosa cells, and cumulus cells. The clustering of the transcriptomes showed the trajectory to the two distinct development courses of oocytes and the surrounding somatic cells. Gene expression changes precipitously increased at Type 4 stage and drastically droped afterwards within both oocytes and granulosa cells. Moreover, the number of differentially expressed genes between oocytes and granulosa cells dramatically increased at Type 4 stage, most of which persistently passed on to the later stages. Strikingly, cell communications within and between oocytes and granulosa cells became active from Type 4 onwards. Cell dialogs connected oocytes and granulosa cells in both unidirectional and bidirectional manners. TGFB2/3, TGFBR2/3, INHBA/B, and ACVR1/1B/2B of TGF-β signaling pathway functioned in the follicle development. NOTCH signaling pathway regulated the development of granulosa cells. Additionally, many maternally DNA methylation- or H3K27me3-imprinted genes remained active in granulosa cells but silent in oocytes during oogenesis. Collectively, Type 4 is the key turning point when significant transcription changes diverge the fate of oocytes and granulosa cells, and the cell dialogs become active to assure follicle development. These findings shed new insights into transcriptomic dynamics and cell dialogs facilitating the development and maturation of oocytes and follicles.
小鼠卵泡发育过程中卵母细胞和颗粒细胞之间的转录组动态和细胞对话
摘要 卵泡的发育和成熟是一个复杂的多阶段过程。卵母细胞和周围体细胞的动态基因表达以及这些细胞之间的对话对这一过程至关重要。我们准确地将卵泡发育划分为九个阶段,并对小鼠卵母细胞、伴随的颗粒细胞和积层细胞的基因表达进行了分析。转录组的聚类显示了卵母细胞和周围体细胞两个不同发育过程的轨迹。在卵母细胞和颗粒细胞中,基因表达的变化在第4型阶段急剧增加,之后又急剧下降。此外,卵母细胞和颗粒细胞之间差异表达基因的数量在第 4 型阶段急剧增加,其中大部分持续到后期阶段。引人注目的是,从第四型开始,卵母细胞和颗粒细胞内部和之间的细胞通讯开始活跃起来。细胞对话以单向和双向方式连接卵母细胞和颗粒细胞。TGF-β信号通路中的TGFB2/3、TGFBR2/3、INHBA/B和ACVR1/1B/2B在卵泡发育中起作用。NOTCH信号通路调控颗粒细胞的发育。此外,在卵子发生过程中,许多母源DNA甲基化或H3K27me3印迹基因在颗粒细胞中保持活性,但在卵母细胞中却沉默不语。总之,第4型是一个关键的转折点,在这一时期,卵母细胞和颗粒细胞的命运发生了重大转录变化,细胞对话变得活跃,从而确保了卵泡的发育。这些发现揭示了促进卵母细胞和卵泡发育和成熟的转录组动态和细胞对话的新见解。
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来源期刊
Genomics, Proteomics & Bioinformatics
Genomics, Proteomics & Bioinformatics Biochemistry, Genetics and Molecular Biology-Biochemistry
CiteScore
14.30
自引率
4.20%
发文量
844
审稿时长
61 days
期刊介绍: Genomics, Proteomics and Bioinformatics (GPB) is the official journal of the Beijing Institute of Genomics, Chinese Academy of Sciences / China National Center for Bioinformation and Genetics Society of China. It aims to disseminate new developments in the field of omics and bioinformatics, publish high-quality discoveries quickly, and promote open access and online publication. GPB welcomes submissions in all areas of life science, biology, and biomedicine, with a focus on large data acquisition, analysis, and curation. Manuscripts covering omics and related bioinformatics topics are particularly encouraged. GPB is indexed/abstracted by PubMed/MEDLINE, PubMed Central, Scopus, BIOSIS Previews, Chemical Abstracts, CSCD, among others.
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