Seed tuber imprinting shapes the next-generation potato microbiome

IF 6.2 2区 环境科学与生态学 Q1 GENETICS & HEREDITY
Yang Song, Jelle Spooren, Casper D. Jongekrijg, Ellen J. H. H. Manders, Ronnie de Jonge, Corné M. J. Pieterse, Peter A. H. M. Bakker, Roeland L. Berendsen
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Abstract

Potato seed tubers are colonized and inhabited by soil-borne microbes, that can affect the performance of the emerging daughter plant in the next season. In this study, we investigated the intergenerational inheritance of microbiota from seed tubers to next-season daughter plants under field condition by amplicon sequencing of bacterial and fungal microbiota associated with tubers and roots, and tracked the microbial transmission from different seed tuber compartments to sprouts. We observed that field of production and potato genotype significantly (P < 0.01) affected the composition of the seed tuber microbiome and that these differences persisted during winter storage of the seed tubers. Remarkably, when seed tubers from different production fields were planted in a single trial field, the microbiomes of daughter tubers and roots of the emerging plants could still be distinguished (P < 0.01) according to the production field of the seed tuber. Surprisingly, we found little vertical inheritance of field-unique microbes from the seed tuber to the daughter tubers and roots, constituting less than 0.2% of their respective microbial communities. However, under controlled conditions, around 98% of the sprout microbiome was found to originate from the seed tuber and had retained their field-specific patterns. The field of production shapes the microbiome of seed tubers, emerging potato plants and even the microbiome of newly formed daughter tubers. Different compartments of seed tubers harbor distinct microbiomes. Both bacteria and fungi on seed tubers have the potential of being vertically transmitted to the sprouts, and the sprout subsequently promotes proliferation of a select number of microbes from the seed tuber. Recognizing the role of plant microbiomes in plant health, the initial microbiome of seed tubers specifically or planting materials in general is an overlooked trait. Elucidating the relative importance of the initial microbiome and the mechanisms by which the origin of planting materials affect microbiome assembly will pave the way for the development of microbiome-based predictive models that may predict the quality of seed tuber lots, ultimately facilitating microbiome-improved potato cultivation.
块茎种子印记塑造下一代马铃薯微生物群
马铃薯块茎种子被土壤中的微生物定殖和栖息,这些微生物会影响下一季子株的生长表现。在本研究中,我们通过对块茎和根部相关的细菌和真菌微生物群进行扩增子测序,研究了在田间条件下块茎种子微生物群对下一季子株的代际遗传,并追踪了微生物从不同块茎种子区系向芽苗的传播。我们观察到,产地和马铃薯基因型对块茎种子微生物群的组成有显著影响(P < 0.01),而且这些差异在块茎种子冬季储藏期间持续存在。值得注意的是,当来自不同产地的块茎种子被种植在同一块试验田中时,子块茎和新植株根部的微生物组仍可根据块茎种子的产地加以区分(P < 0.01)。令人惊讶的是,我们发现从块茎种子到子块茎和根部的田间独特微生物几乎没有垂直遗传,只占各自微生物群落的不到 0.2%。然而,在受控条件下,我们发现约 98% 的芽微生物群落源自块茎种子,并保留了其田间特有的模式。生产领域决定着块茎种子、新生马铃薯植株的微生物群落,甚至是新形成的子块茎的微生物群落。块茎种子的不同区域蕴藏着不同的微生物群。块茎种子上的细菌和真菌都有可能垂直传播到萌芽上,萌芽随后会促进块茎种子上精选微生物的增殖。认识到植物微生物群在植物健康中的作用,块茎种子或一般种植材料的初始微生物群是一个被忽视的特性。阐明初始微生物组的相对重要性以及种植材料来源影响微生物组组合的机制,将为开发基于微生物组的预测模型铺平道路,该模型可预测块茎种子批次的质量,最终促进微生物改良马铃薯栽培。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Environmental Microbiome
Environmental Microbiome Immunology and Microbiology-Microbiology
CiteScore
7.40
自引率
2.50%
发文量
55
审稿时长
13 weeks
期刊介绍: Microorganisms, omnipresent across Earth's diverse environments, play a crucial role in adapting to external changes, influencing Earth's systems and cycles, and contributing significantly to agricultural practices. Through applied microbiology, they offer solutions to various everyday needs. Environmental Microbiome recognizes the universal presence and significance of microorganisms, inviting submissions that explore the diverse facets of environmental and applied microbiological research.
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