Genome analysis of three isolates of Stemphylium lycopersici differ in their virulence and sporulation ability: Identification of effectors, pathogenesis and virulence factors

IF 2.3 3区农林科学 Q1 AGRONOMY

Plant Pathology Pub Date : 2024-02-17 DOI:10.1111/ppa.13878

Rocío Medina, Mario E. E. Franco, Laura C. Bartel, Cesar G. Lucentini, Silvina M. Y. López Diaz, Mario C. N. Saparrat, Pedro A. Balatti

{"title":"Genome analysis of three isolates of Stemphylium lycopersici differ in their virulence and sporulation ability: Identification of effectors, pathogenesis and virulence factors","authors":"Rocío Medina, Mario E. E. Franco, Laura C. Bartel, Cesar G. Lucentini, Silvina M. Y. López Diaz, Mario C. N. Saparrat, Pedro A. Balatti","doi":"10.1111/ppa.13878","DOIUrl":null,"url":null,"abstract":"Tomato grey leaf spot is a fungal disease that provokes losses in tomato yield. The aim of this work is to analyse genomic differences among three isolates of Stemphylium lycopersici that differ in virulence and sporulation. The bioinformatics analysis led us to predict the identity of putative effectors, pathogenesis and virulence factors. Like the genome of other necrotrophic pathogens, Stemphylium encodes a wide spectrum of effectors, including an ample and diverse array of carbohydrate-degrading enzymes. Interestingly, the number of predicted effectors was unrelated to virulence. Low virulence appeared to be associated with the presence of several double-stranded RNAs from viruses as well as cellular processes related to protein degradation, redox and detoxifying processes and monoterpenes production. This is the first identification of the potential effectors of tomato–S. lycopersici interaction; nonetheless functional studies should be done.","PeriodicalId":20075,"journal":{"name":"Plant Pathology","volume":"75 1","pages":""},"PeriodicalIF":2.3000,"publicationDate":"2024-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Pathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1111/ppa.13878","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}

引用次数: 0

Abstract

Tomato grey leaf spot is a fungal disease that provokes losses in tomato yield. The aim of this work is to analyse genomic differences among three isolates of Stemphylium lycopersici that differ in virulence and sporulation. The bioinformatics analysis led us to predict the identity of putative effectors, pathogenesis and virulence factors. Like the genome of other necrotrophic pathogens, Stemphylium encodes a wide spectrum of effectors, including an ample and diverse array of carbohydrate-degrading enzymes. Interestingly, the number of predicted effectors was unrelated to virulence. Low virulence appeared to be associated with the presence of several double-stranded RNAs from viruses as well as cellular processes related to protein degradation, redox and detoxifying processes and monoterpenes production. This is the first identification of the potential effectors of tomato–S. lycopersici interaction; nonetheless functional studies should be done.

Abstract Image

查看原文本刊更多论文

对 Stemphylium lycopersici 的三种分离株进行基因组分析，发现它们的毒力和孢子繁殖能力各不相同：效应因子、致病机理和毒力因子的鉴定

番茄灰叶斑病是一种真菌病害，会导致番茄减产。这项工作的目的是分析 Stemphylium lycopersici 三种分离物之间的基因组差异，这些分离物在毒力和孢子生成方面存在差异。通过生物信息学分析，我们预测了假定效应因子、致病机理和毒力因子的特性。与其他坏死性病原体的基因组一样，Stemphylium 也编码多种效应因子，包括大量不同的碳水化合物降解酶。有趣的是，预测效应物的数量与毒力无关。低毒性似乎与存在几种来自病毒的双链 RNA 以及与蛋白质降解、氧化还原和解毒过程以及单萜生产相关的细胞过程有关。这是对番茄-S. lycopersici 相互作用潜在效应因子的首次鉴定，但仍需进行功能性研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Plant Pathology 生物-农艺学

CiteScore

5.60

自引率

7.40%

发文量

147

审稿时长

3 months

期刊介绍： This international journal, owned and edited by the British Society for Plant Pathology, covers all aspects of plant pathology and reaches subscribers in 80 countries. Top quality original research papers and critical reviews from around the world cover: diseases of temperate and tropical plants caused by fungi, bacteria, viruses, phytoplasmas and nematodes; physiological, biochemical, molecular, ecological, genetic and economic aspects of plant pathology; disease epidemiology and modelling; disease appraisal and crop loss assessment; and plant disease control and disease-related crop management.