Transcriptome profiling and characterization of genes associated with tuberization under high temperature in aeroponics in potato cv. Kufri Anand.

IF 1.6 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Genes & genomics Pub Date : 2024-04-01 Epub Date: 2024-02-21 DOI:10.1007/s13258-024-01503-z
Rasna Zinta, Jagesh Kumar Tiwari, Tanuja Buckseth, Umesh Goutam, Rajesh Kumar Singh, Vinod Kumar, Ajay Kumar Thakur
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引用次数: 0

Abstract

Background: High temperature stress is an important abiotic factor, which affects tuberization and ultimately causes heavy yield reduction in potato.

Objectives: Identification and characterization of genes associated with tuberization under high temperature stress is essential for future management through biotechnology.

Methodology: Two contrasting potato varieties Kufri Anand (profuse tuber-bearing) versus Kufri Frysona (very less/scanty tuber-bearing, control) were cultivated in aeroponics under high temperature stress, and transcriptomes were analyzed.

Results: Potato cv. Kufri Anand was found superior over control (Kufri Frysona) for tuber yield and its component traits along with root morphology under aeroponics. Transcriptomes of tuber and leaf tissues were analyzed. Statistically significant (p < 0.05) differentially expressed genes (DEGs) were categorised into up-regulated (> 2 log2 fold change, FC) and down-regulated (< -2 log2 FC) genes. DEGs were annotated by gene ontology and KEGG pathways. A few selected up-regulated genes of both tissues were identified, and phylogeny tree and motif analysis were analysed based on 36 peptide sequences representing 15 selected DEGs in this study. Further, gene expression markers were developed and validated by real time qPCR analysis for the identification of high temperature tolerant genotypes.

Conclusion: A few key genes associated in tuberization under high temperature conditions were heat shock proteins (e.g. 18.5 kDa class I heat shock protein), sugar metabolism (e.g. glucosyltransferase), transcription factor (e.g. WRKY), and phytohormones (e.g. auxin-induced beta-glucosidase). Our study provides an overview of key genes involved in tuberization under high temperature stress in potato cv. Kufri Anand under aeroponics.

马铃薯变种 Kufri Anand 在气培高温条件下块茎化相关基因的转录组特征分析库夫里-阿南德
背景:高温胁迫是一个重要的非生物因素,它会影响马铃薯的块茎化,并最终导致马铃薯严重减产:高温胁迫下块茎化相关基因的鉴定和特征描述对于未来通过生物技术进行管理至关重要:方法:在高温胁迫下用气生栽培法培育了两个不同的马铃薯品种Kufri Anand(结块茎多)和Kufri Frysona(结块茎少/稀疏,对照),并对转录组进行了分析:结果:马铃薯品种结果发现,在气培条件下,Kufri Anand 马铃薯变种的块茎产量及其组成性状以及根系形态均优于对照(Kufri Frysona)。对块茎和叶片组织的转录组进行了分析。结果表明,在气培条件下,与块茎和叶片组织相关的几个关键基因的转录组具有统计学意义(p 2 log2 折合变化,FC)和下调作用(结论:在气培条件下,与块茎和叶片组织相关的几个关键基因的转录组具有统计学意义(p 2 log2 折合变化,FC):高温条件下与块茎化相关的几个关键基因是热休克蛋白(如 18.5 kDa I 类热休克蛋白)、糖代谢(如葡萄糖基转移酶)、转录因子(如 WRKY)和植物激素(如辅助素诱导的 beta-葡萄糖苷酶)。我们的研究概述了在高温胁迫下参与马铃薯变种 Kufri Anand 块茎化的关键基因。Kufri Anand 在气培条件下块茎化的关键基因。
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来源期刊
Genes & genomics
Genes & genomics 生物-生化与分子生物学
CiteScore
3.70
自引率
4.80%
发文量
131
审稿时长
6-12 weeks
期刊介绍: Genes & Genomics is an official journal of the Korean Genetics Society (http://kgenetics.or.kr/). Although it is an official publication of the Genetics Society of Korea, membership of the Society is not required for contributors. It is a peer-reviewed international journal publishing print (ISSN 1976-9571) and online version (E-ISSN 2092-9293). It covers all disciplines of genetics and genomics from prokaryotes to eukaryotes from fundamental heredity to molecular aspects. The articles can be reviews, research articles, and short communications.
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