T Lymphocyte Interferon-gamma Response to Anaplasmataceae-related Major Surface Proteins and Ankyrin A in Fibromyalgia.

CNS & neurological disorders drug targets Pub Date : 2024-01-01 DOI:10.2174/0118715273274091231207101522

Basant K Puri, Rosemarie Preyer, Gary S Lee, Armin Schwarzbach

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Abstract

Background: The aetiology of fibromyalgia is unknown; its symptoms may be related to a T-lymphocyte-mediated response to infectious organisms.

Objectives: First, to test the hypothesis that fibromyalgia is associated with increased interferon (IFN)-γ-secreting T-lymphocytes after stimulation with Anaplasmataceae-related major surface proteins (MSPs) and the macromolecular translocation type IV secretion system effector ankyrin repeat domain-containing protein A (AnkA). Second, to ascertain the relationship in fibromyalgia between (i) the IFN-γ-secreting T-lymphocyte response to stimulation with Anaplasmataceae-related MSPs and AnkA, and (ii) co-infection by Borrelia and Yersinia spp., and antinuclear antibodies.

Methods: Using a case-control design, patients fulfilling the American College of Rheumatology revised criteria for fibromyalgia, and controls, underwent the following blinded assessments: (i) enzyme- linked immune absorbent spot (ELISpot) IFN-γ release assay of T-lymphocyte reactivity to Anaplasmataceae-related MSPs and AnkA; (ii) ELISpot IFN-γ release assays of T-lymphocyte reactivity to three Borrelia antigens, namely Borrelia burgdorferi full antigen (B31); peptide mix (from Borrelia burgdorferi sensu stricto, Borrelia afzelii, Borrelia garinii); and Borrelia burgdorferi lymphocyte function-associated antigen-1; (iii) immunoglobulin (Ig) A assay by enzyme-linked immunosorbent assay (ELISA) of antibodies to Yersinia spp.; (iv) IgG (ELISA) antibodies to Yersinia spp.; (v) serum antinuclear antibodies (immunofluorescence).

Results: The groups were age- and sex-matched. The mean (standard error) value of IFN-γ release for the fibromyalgia group was 1.52 (0.26), compared with 1.00 (0.22) for the controls. Generalised linear modelling (p<0.001) of IFN-γ release in the fibromyalgia patients showed significant main effects of all three indices of Borrelia infection and of antinuclear antibodies.

Conclusion: Anaplasmataceae may play an aetiological role in fibromyalgia.

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T淋巴细胞干扰素-γ对纤维肌痛中与疟原虫相关的主要表面蛋白和Ankyrin A的反应

背景：纤维肌痛的病因不明，其症状可能与T淋巴细胞介导的对感染性生物体的反应有关：首先，验证纤维肌痛与干扰素（IFN）-γ分泌增加的T淋巴细胞在受到与无鞭毛疟原虫相关的主要表面蛋白（MSFs）和大分子转位IV型分泌系统效应物含ankrin重复域蛋白A（AnkA）刺激后有关的假设。其次，确定纤维肌痛中(i)分泌 IFN-γ 的 T 淋巴细胞对无鞭毛虫相关 MSFs 和 AnkA 刺激的反应，以及(ii)包柔氏和耶尔森氏菌属的合并感染和抗核抗体之间的关系：采用病例对照设计，对符合美国风湿病学会纤维肌痛修订标准的患者和对照组进行以下盲法评估：(i)酶联免疫吸附斑（ELISpot）IFN-γ释放试验，检测T淋巴细胞对无鞭毛疟原虫相关MSFs和AnkA的反应性；(ii)ELISpot IFN-γ释放试验，检测T淋巴细胞对三种包柔氏菌抗原的反应性，即包柔氏菌全抗原（B31）；(iii)通过酶联免疫吸附试验（ELISA）检测耶尔森氏菌属抗体的免疫球蛋白（Ig）A；(iv)通过酶联免疫吸附试验（ELISA）检测耶尔森氏菌属抗体的免疫球蛋白（IgG）；(viii)通过酶联免疫吸附试验（ELISA）检测耶尔森氏菌属抗体的免疫球蛋白（Ig）A；(viii)通过酶联免疫吸附试验（ELISA）检测耶尔森氏菌属抗体的免疫球蛋白（Ig）A。结果：各组的年龄和性别均匹配。纤维肌痛组的 IFN-γ 释放平均值（标准误差）为 1.52（0.26），而对照组为 1.00（0.22）。广义线性模型（pConclusion：疟原虫可能是纤维肌痛的病因之一。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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CNS & neurological disorders drug targets

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