Cellobiose dehydrogenase (CDH) plays a crucial role in lignocellulose degradation and bioelectrochemical industries, making it highly in demand. However, the production and purification of CDH through fungal heterologous expression methods is time-consuming, costly, and challenging. In this study, we successfully displayed Pycnoporus sanguineus CDH (psCDH) on the surface of Bacillus subtilis spores for the first time. Enzymatic characterization revealed that spore surface display enhanced the tolerance of psCDH to high temperature (80 °C) and low pH levels (3.5) compared to free psCDH. Furthermore, we found that glycerol, lactic acid, and malic acid promoted the activity of immobilized spore-displayed psCDH; glycerol has a more significant stimulating effect, increasing the activity from 16.86 ± 1.27 U/mL to 46.26 ± 3.25 U/mL. After four reuse cycles, the psCDH immobilized with spores retained 48% of its initial activity, demonstrating a substantial recovery rate. In conclusion, the spore display system, relying on cotG, enables the expression and immobilization of CDH while enhancing its resistance to adverse conditions. This system demonstrates efficient enzyme recovery and reuse. This approach provides a novel method and strategy for the immobilization and stability enhancement of CDH.
公司名称 | 产品信息 | 采购帮参考价格 |
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阿拉丁 |
Isopropyl-β-D-thiogalactoside (IPTG)
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¥16.00~¥328809.66 |
阿拉丁 |
Glycerol
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¥18.00~¥65605.20 |
阿拉丁 |
sodium fluoride
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¥20.00~¥29147.72 |
上海源叶 |
cellobiose
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¥35.00~¥20028.00 |
阿拉丁 |
2,6-dichlorophenolindophenol (DCIP)
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¥29.00~¥15555.00 |
阿拉丁 |
Salicylic Acid (SA)
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¥10.00~¥11565.44 |
阿拉丁 |
Ethylenediamine Tetraacetic Acid (EDTA)
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¥4.00~¥6172.00 |
阿拉丁 |
Dimethyl Phenol (DPM)
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阿拉丁 |
Copper ion (Cu2?)
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阿拉丁 |
Lactic Acid (DLA)
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阿拉丁 |
Potassium ion (K?)
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阿拉丁 |
Nitrophenol (PNP)
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阿拉丁 |
Malic Acid (MA)
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阿拉丁 |
cytochrome C (Cyt C)
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阿拉丁 |
Guaiacol (Gua)
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阿拉丁 |
Succinic Acid (SuA)
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