Alleviating effect of Lactobacillus pentosus CQZC01 probiotic strain on antibiotic-induced diarrhea in mice

Yanqing Liu, Mengqin Cheng, Bihui Liu, Mengwei Wang, Jing Song, Xin Zhao, Ruokun Yi, X. Long, Huazhi Liu
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Abstract

This experiment used mice as the experimental subjects to study the alleviating effect of Lactobacillus pentosus CQZC01 (LPCQZC01) on antibiotic-induced diarrhea in mice. After adaptive feeding for seven days, Kunming (KM) mice were randomly divided into the following groups: positive control group, model group, normal group, high concentration group of LPCQZC01 (H-LPCQZC01, 1×109 CFU/mL), and low concentration group of LPCQZC01 (L-LPCQZC01, 1×108 CFU/mL). Except for the normal group, mice were orally administered with lincomycin hydrochloride (120 mg/day) for 7 consecutive days. Changes in body weight, food intake, water consumption, and fecal water content of mice were observed. A histological examination of mouse colon and small intestine sections was done to find out if Lactobacillus pentosus CQZC01 may help mice with antibiotic-induced diarrhea. The findings demonstrated that, in comparison to the model group, mice in the H-LPCQZC01 and L-LPCQZC01 groups had considerably lower blood levels of nterleukin-6 (IL-6), interleukin-17A (IL-17A), 5-hydroxytryptamine (5-HT), and malondialdehyde (MDA), and other variables. In comparison to the model group, the fecal water content in the H-LPCQZC01 and L-LPCQZC01 groups was considerably lower. The H-LPCQZC01 and L-LPCQZC01 groups consumed more food and liquids than the model group did. The H-LPCQZC01 and L-LPCQZC01 groups had more intact colon walls and more compact, regular, and organized intestinal villi. H-LPCQZC01 and L-LPCQZC01 groups had the lower CFTR, EGFR expression and stronger NHE1, NHE2 expression than model group. This study reveals that Lactobacillus pentosus CQZC01 may treat inflammatory lesions in the mouse intestines and successfully treat antibiotic-induced diarrhea in mice.
戊糖乳杆菌 CQZC01 益生菌株对抗生素引起的小鼠腹泻的缓解作用
本实验以小鼠为实验对象,研究五味子乳杆菌 CQZC01(LPCQZC01)对抗生素引起的小鼠腹泻的缓解作用。昆明(KM)小鼠经七天适应性喂养后,随机分为以下几组:阳性对照组、模型组、正常组、LPCQZC01高浓度组(H-LPCQZC01,1×109 CFU/mL)和LPCQZC01低浓度组(L-LPCQZC01,1×108 CFU/mL)。除正常组外,小鼠连续7天口服盐酸林可霉素(120毫克/天)。观察小鼠体重、进食量、饮水量和粪便含水量的变化。对小鼠结肠和小肠切片进行了组织学检查,以确定五胜肽乳杆菌 CQZC01 是否有助于治疗抗生素引起的小鼠腹泻。研究结果表明,与模型组相比,H-LPCQZC01 组和 L-LPCQZC01 组小鼠血液中的白细胞介素-6(IL-6)、白细胞介素-17A(IL-17A)、5-羟色胺(5-HT)、丙二醛(MDA)及其他变量的水平均显著降低。与模型组相比,H-LPCQZC01组和L-LPCQZC01组的粪便含水量要低得多。与模型组相比,H-LPCQZC01 和 L-LPCQZC01 组消耗了更多的食物和液体。H-LPCQZC01组和L-LPCQZC01组的结肠壁更完整,肠绒毛更紧密、规则和有序。与模型组相比,H-LPCQZC01 组和 L-LPCQZC01 组的 CFTR 和 EGFR 表达较低,NHE1 和 NHE2 表达较高。这项研究揭示了五味子乳杆菌 CQZC01 可治疗小鼠肠道的炎症病变,并成功治疗抗生素引起的小鼠腹泻。
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