Content of selected polyphenolic compounds in four Sambucus nigra l. cultivars in relation to their antimicrobial activity

Acta Poloniae Pharmaceutica - Drug Research Pub Date : 2024-02-01 DOI:10.32383/appdr/175051

I. Korona-Głowniak, Martyna Kasela, Barbara Pliszka, D. Zalewski, Anna Malm

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Abstract

European elderberry (Sambucus nigra L.) fruits constitute a valuable source of biologically active compounds useful in the pharmaceutical industry. We aimed to determine the content of chosen polyphenolic compounds in fruit extracts from four cultivars of elderberry (Alleso, Korsor, Sampo, Samyl) and their relation to antibacterial and antifungal activity. The content of polyphenols was determined with the use of high-performance liquid chromatography (HPLC), while antimicrobial activity, including minimum inhibitory concentration (MIC), minimum bactericidal (MBC) or fungicidal (MFC) concentration, was studied with the use of microbroth dilution method. HPLC analysis revealed the presence of concentrations of cyanidin glycosides, caffeic and quercetin derivatives depending on the plant cultivar. The extracts exhibited the highest antibacterial activity against Staphylococcus spp. (MIC = 0.313 – 0.625 mg/mL) and Helicobacter pylori (MIC = 0.313 – 1.25 mg/mL) and antifungal against Candida albicans and C. parapsilosis (MIC = 0.313 – 2.5 mg/mL). Results showed that the concentration of cyanidin glycosides, caffeic and quercetin derivatives depended on the analyzed S. nigra L. cultivar and pointed towards a correlation between their high content and antimicrobial activity. These results support the idea that elderberry fruits contain bioactive compounds providing them significant antimicrobial potential.

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四种黑三叶草栽培品种中某些多酚化合物的含量与其抗菌活性的关系

欧洲接骨木（Sambucus nigra L.）果实是制药业有用的生物活性化合物的宝贵来源。我们的目的是测定四种接骨木（Alleso、Korsor、Sampo、Samyl）果实提取物中精选多酚化合物的含量及其与抗菌和抗真菌活性的关系。使用高效液相色谱法（HPLC）测定了多酚类化合物的含量，并使用微流稀释法研究了抗菌活性，包括最低抑菌浓度（MIC）、最低杀菌浓度（MBC）或杀真菌浓度（MFC）。高效液相色谱分析显示，根据植物品种的不同，青花素苷、咖啡酸和槲皮素衍生物的浓度也不同。提取物对葡萄球菌属（MIC = 0.313 - 0.625 mg/mL）和幽门螺旋杆菌（MIC = 0.313 - 1.25 mg/mL）的抗菌活性最高，对白色念珠菌和副丝状芽孢杆菌（MIC = 0.313 - 2.5 mg/mL）的抗真菌活性最高。结果表明，花青素苷、咖啡酸和槲皮素衍生物的浓度取决于所分析的黑接骨木栽培品种，并指出它们的高含量与抗菌活性之间存在相关性。这些结果支持了接骨木果实含有生物活性化合物的观点，使其具有显著的抗菌潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Acta Poloniae Pharmaceutica - Drug Research

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