Optimization of Pulsed Electric Field-Based Extraction of Bioactive Compounds from Cannabis sativa Leaves

Analytica Pub Date : 2024-02-04 DOI:10.3390/analytica5010006

Dimitrios Mpakos, Theodoros G. Chatzimitakos, V. Athanasiadis, Martha Mantiniotou, Eleni Bozinou, S. Lalas

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Abstract

The current investigation examines the application of pulsed electric fields (PEFs) for isolating polyphenols from Cannabis sativa var. Futura 75 leaves. Firstly, the solvent composition, which included ethanol, water, and various mixtures of the two, was explored, along with the liquid-to-solid ratio. Subsequently, the primary parameters associated with PEFs (namely, pulse duration, pulse period, electric field intensity, and treatment duration) were optimized. The extracted samples were analyzed to determine their total polyphenol content (TPC), and individual polyphenols were also evaluated through high-performance liquid chromatography. In addition, the antioxidant activity of the extracts was assessed through ferric-reducing antioxidant power (FRAP) and DPPH assays. The extracts prepared utilizing PEFs were compared to the extracts obtained without PEFs in terms of their TPC, FRAP values, and DPPH activity. The results indicate that the most effective extraction parameters were a pulse duration of 10 μs, a pulse period of 1000 μs, and an electric field strength of 0.9 kV/cm after 25 min of extraction. The most efficient solvent was determined to be a 50% (v/v) mixture of ethanol and water in a 20:1 liquid-to-solid ratio. The extract obtained under the optimal conditions exhibited a ~75% increase in TPC compared to the extract obtained without any application of PEFs, while some individual polyphenols exhibited an increase of up to ~300%. Furthermore, significant increases of ~74% and ~71% were observed in FRAP and DPPH assays. From the information provided, it was observed that the tested variables had an impact on the recovery of polyphenols from C. sativa leaves.

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优化基于脉冲电场的大麻叶生物活性化合物提取方法

目前的研究探讨了脉冲电场 (PEF) 在从大麻变种 Futura 75 叶片中分离多酚中的应用。首先，研究了溶剂成分（包括乙醇、水和二者的各种混合物）以及液固比。随后，对与 PEF 相关的主要参数（即脉冲持续时间、脉冲周期、电场强度和处理持续时间）进行了优化。对提取的样品进行了分析，以确定其多酚总含量（TPC），并通过高效液相色谱法对单个多酚进行了评估。此外，还通过铁还原抗氧化能力（FRAP）和 DPPH 试验评估了提取物的抗氧化活性。将使用 PEFs 制备的提取物与不使用 PEFs 的提取物在 TPC、FRAP 值和 DPPH 活性方面进行了比较。结果表明，萃取 25 分钟后，最有效的萃取参数是脉冲持续时间 10 μs、脉冲周期 1000 μs、电场强度 0.9 kV/cm。最有效的溶剂被确定为液固比为 20:1 的 50%（v/v）乙醇和水的混合物。与未使用任何 PEFs 的提取物相比，在最佳条件下获得的提取物的 TPC 增加了约 75%，而某些单个多酚的增幅高达约 300%。此外，在 FRAP 和 DPPH 检测中也观察到了 ~74% 和 ~71% 的明显增加。从所提供的信息中可以看出，所测试的变量对从荠菜叶中回收多酚有影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Analytica

CiteScore

1.80

自引率

0.00%

发文量