Effect of Latanoprostene Bunod on the Permeability of Trabecular Meshwork Cells

IF 0.1 Q4 OPHTHALMOLOGY

Journal of The Korean Ophthalmological Society Pub Date : 2024-02-15 DOI:10.3341/jkos.2024.65.2.139

Hyeong Seok Park, Jae Woo Kim

{"title":"Effect of Latanoprostene Bunod on the Permeability of Trabecular Meshwork Cells","authors":"Hyeong Seok Park, Jae Woo Kim","doi":"10.3341/jkos.2024.65.2.139","DOIUrl":null,"url":null,"abstract":"Purpose: To investigate the effects of latanoprostene bunod (LBN) on nitric oxide (NO) production and permeability in human trabecular meshwork cells (HTMC).Methods: HTMC were treated with 50 and 100 µM LBN and latanoprost free acid (LAT) for 30 minutes. Additionally, 100 µM LBN was co-exposed to 0.5 mM L-NAME (N-Nitroarginine methyl ester). Cellular viability and NO production were measured using MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) and Griess assays. The permeability and resistance of the HTMC monolayer were evaluated by trans-endothelial electrical resistance (TEER) and carboxyfluorescein permeability.Results: Exposure to 100 µM LBN led to increased NO production, whereas co-exposure to L-NAME reduced NO production. Treatment with 100 µM LBN decreased the TEER of the HTMC monolayer. LBN exposure heightened carboxyfluorescein permeability, but co-exposure to 100 µM LBN and L-NAME reduced permeability. LAT treatment did not affect NO production or permeability.Conclusions: LBN increased the permeability of the HTMC monolayer and increased NO production. Therefore, LBN might increase trabecular outflow in addition to promoting uveoscleral outflow.","PeriodicalId":17341,"journal":{"name":"Journal of The Korean Ophthalmological Society","volume":null,"pages":null},"PeriodicalIF":0.1000,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of The Korean Ophthalmological Society","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3341/jkos.2024.65.2.139","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"OPHTHALMOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Purpose: To investigate the effects of latanoprostene bunod (LBN) on nitric oxide (NO) production and permeability in human trabecular meshwork cells (HTMC).Methods: HTMC were treated with 50 and 100 µM LBN and latanoprost free acid (LAT) for 30 minutes. Additionally, 100 µM LBN was co-exposed to 0.5 mM L-NAME (N-Nitroarginine methyl ester). Cellular viability and NO production were measured using MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) and Griess assays. The permeability and resistance of the HTMC monolayer were evaluated by trans-endothelial electrical resistance (TEER) and carboxyfluorescein permeability.Results: Exposure to 100 µM LBN led to increased NO production, whereas co-exposure to L-NAME reduced NO production. Treatment with 100 µM LBN decreased the TEER of the HTMC monolayer. LBN exposure heightened carboxyfluorescein permeability, but co-exposure to 100 µM LBN and L-NAME reduced permeability. LAT treatment did not affect NO production or permeability.Conclusions: LBN increased the permeability of the HTMC monolayer and increased NO production. Therefore, LBN might increase trabecular outflow in addition to promoting uveoscleral outflow.

查看原文本刊更多论文

拉坦前列腺素布诺对小梁网细胞渗透性的影响

目的：研究拉坦前列腺素布诺（LBN）对人小梁网状细胞（HTMC）一氧化氮（NO）产生和通透性的影响。方法：用 50 µM 和 100 µM LBN 和游离酸（LAT）处理 HTMC 30 分钟。此外，100 µM LBN 与 0.5 mM L-NAME（N-硝基精氨酸甲酯）同时暴露。使用 MTT（3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium溴化物）和 Griess 试验测定细胞活力和 NO 的产生。通过跨内皮电阻（TEER）和羧基荧光素渗透性评估了 HTMC 单层的渗透性和电阻：结果：暴露于 100 µM LBN 会导致 NO 生成增加，而同时暴露于 L-NAME 则会减少 NO 生成。100 µM LBN 可降低 HTMC 单层的 TEER。暴露于 LBN 会增加羧基荧光素的通透性，但同时暴露于 100 µM LBN 和 L-NAME 会降低通透性。LAT 处理不会影响 NO 的产生或通透性：结论：LBN 增加了 HTMC 单层的通透性并增加了 NO 的产生。因此，除了促进葡萄膜巩膜外流外，LBN 还可能增加小梁外流。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of The Korean Ophthalmological Society OPHTHALMOLOGY-

CiteScore

0.20

自引率

0.00%

发文量

126