Synthesis, characterization, and bioactivity test of dibutyltin(IV) dihydroxyibenzoate as disinfectant agent

Abstract

In this work, three organotin (IV) derivatives namely dibutyltin(IV) di-2-hydroxybenzoate (DBT2-HBz) (2), dibutyltin(IV) di-3-hydroxybenzoate (DBT3-HBz) (3) and dibutyltin(IV) di-4-hydroxybenzoate (DBT4-HBz)) (4) were synthesized and tested as disinfectant against pathogenic bacteria, Staphylococcus aureus. Compounds 2–4 were prepared by reacting dibutyltin(IV) oxide (DBTO) (1) with 2-hydroxybenzoic acid (2-HHBz), 3-hydroxybenzoic acid (3-HHBz) and 4-hydroxybenzoic acid (4-HHBz). The synthesized compounds were characterized by spectroscopic techniques including UV–Vis, FT-IR, 1H NMR, and 13C NMR, and micro-elemental analyzer. The optical density (OD) of compounds was measured by UV–Vis at 600 nm, which showed good bioactivity against the S. aureus. The data obtained indicated that compound 4 has higher bioactivity than compound 2 and 3, and the positive control using a commercial disinfectant containing 5 % benzalkonium chloride. The optimum bioactivity of compound 4 was observed at a concentration of 5 × 10−4 M with a contact time of 10 min, as shown by the decrease in absorbance from 0.6092 to 0.4005. Compound 2 was optimal at a concentration of 5 × 10−4 M with a contact time of 10 min, and the absorbance decreased from 0.6092 to 0.4416. Compound 3 and positive control showed a smaller decrease in absorbance compared to compound 4 and 2. Overall, the results obtained indicated that the compounds synthesized have promising potential as disinfectant against pathogenic bacteria.