Comparison of four inoculation methods and three Fusarium species for phenotyping stalk rot resistance among 22 maize hybrids (Zea mays)

IF 2.3 3区农林科学 Q1 AGRONOMY

Plant Pathology Pub Date : 2024-02-04 DOI:10.1111/ppa.13874

Desmond Darko Asiedu, Félicien Akohoue, Sebastian Frank, Silvia Koch, Bärbel Lieberherr, Benedict Oyiga, Bettina Kessel, Thomas Presterl, Thomas Miedaner

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Abstract

Fusarium stalk rot (FSR) is among the most destructive maize diseases causing significant global yield losses. Resistance of 22 maize hybrids to FSR was tested using four inoculation methods in each of two locations in 2021 and 2022. The inoculation methods included needle injection (NI), toothpick method (TM), stick method (SM) and mycelium method (MM), and the inoculated fungi were Fusarium culmorum, F. graminearum and F. temperatum. NI displayed the highest FSR infection among maize hybrids followed by TM and SM. MM showed the least infection. From five stalk rot-related traits, full-length infection and internode proportion, that is, the percentage of visible infection summed up over internodes, captured most of the genetic variation. The latter was the trait with the highest heritability (0.90). No significant (p > 0.05) genotype × method and genotype × fungus interaction variances were observed for any traits. For F. graminearum inoculation, NI showed the highest internode proportion followed by TM and SM, with F. culmorum responding in a similar way. For F. temperatum, TM outranked all other methods. F. graminearum was the most aggressive fungal pathogen compared to F. culmorum and F. temperatum. For phenotyping maize lines with varying degrees of resistance to FSR, we recommend needle injection and internode proportion.

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比较四种接种方法和三种镰刀菌对 22 个玉米杂交种（玉米）茎腐病抗性的表型作用

镰刀菌茎腐病（Fusarium stalk rot，FSR）是破坏性最强的玉米病害之一，对全球产量造成重大损失。2021 年和 2022 年，在两个地点分别采用四种接种方法测试了 22 个玉米杂交种对茎腐镰刀菌的抗性。接种方法包括针刺法（NI）、牙签法（TM）、棍棒法（SM）和菌丝法（MM），接种的真菌为高秆镰刀菌、禾谷镰刀菌和温疫镰刀菌。在玉米杂交种中，NI 的 FSR 感染率最高，其次是 TM 和 SM。MM 的感染率最低。在五个与茎腐病相关的性状中，全长感染和节间比例（即可见感染在节间所占的百分比总和）占据了遗传变异的大部分。后者是遗传率最高的性状（0.90）。没有观察到基因型×方法和基因型×真菌对任何性状有明显的交互变异（p > 0.05）。对于禾本科真菌接种，NI 表现出最高的节间比例，其次是 TM 和 SM，F. culmorum 的反应类似。对于F. temperatum来说，TM方法优于所有其他方法。与F. culmorum和F. temperatum相比，F. graminearum是最具侵袭性的真菌病原体。对于具有不同程度抗FSR能力的玉米品系的表型，我们建议采用针刺法和节间比例法。

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来源期刊

Plant Pathology 生物-农艺学

CiteScore

5.60

自引率

7.40%

发文量

147

审稿时长

3 months

期刊介绍： This international journal, owned and edited by the British Society for Plant Pathology, covers all aspects of plant pathology and reaches subscribers in 80 countries. Top quality original research papers and critical reviews from around the world cover: diseases of temperate and tropical plants caused by fungi, bacteria, viruses, phytoplasmas and nematodes; physiological, biochemical, molecular, ecological, genetic and economic aspects of plant pathology; disease epidemiology and modelling; disease appraisal and crop loss assessment; and plant disease control and disease-related crop management.