Lipidomics Analysis of Different Marine Fish Oils Using Untargeted Liquid Chromatography–Orbitrap High-Resolution Mass Spectrometry and Chemometrics

IF 1.2 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Chromatographia Pub Date : 2024-02-17 DOI:10.1007/s10337-024-04312-4

Anjar Windarsih, Irnawati, Suratno, Hendy Dwi Warmiko, Lucky Prabowo Miftachul Alam, Indrawati Dian Utami, Abdul Rohman, Anastasia Wheni Indrianingsih

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Abstract

Marine fish oils (MFOs) have been known for their nutritional compounds, which benefit human health. MFOs contain various lipids which play an essential role in human nutrition. The objective of this research was to apply untargeted lipidomics analysis using liquid chromatography–high-resolution mass spectrometry (LC–HRMS) for the comprehensive identification of lipid compositions extracted from five different marine fishes, namely red snapper (Lutjanus campechanus (A1)), giant grouper (Epinephelus lanceolatus (A2)), baronang crochet (Siganus canaliculatus (B2)), white snapper (Lates calcarifer (C3)), skipjack tuna (Katsuwonus pelamis (D2)). More than 1000 lipid compounds from both positive and negative ionization modes could be detected in each MFO using LC–HRMS untargeted lipidomics analysis. Most lipids were dominated by triglycerides (TG) and diglycerides (DG) classes. Principal component analysis (PCA) and partial least square-discriminant analysis (PLS-DA) could classify MFO samples in positive and negative ionization modes. Based on variable importance for projections (VIP) value, 15 differentiating lipid compounds were found to have an essential role as potential biomarkers in positive ionization mode. In contrast, ten differentiating lipid compounds were found in negative ionization mode. It can be concluded that untargeted lipidomics using LC–HRMS could be used as a powerful analytical technique for comprehensively identifying lipid compositions in MFO samples. It can be further used to identify the lipid compositions of other fish oil samples.

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利用非靶向液相色谱-轨道阱高分辨质谱法和化学计量学分析不同海洋鱼油的脂质组学

海洋鱼油（MFOs）因其营养化合物而闻名，对人类健康有益。海洋鱼油含有多种脂质，在人类营养中发挥着重要作用。本研究的目的是利用液相色谱-高分辨质谱法（LC-HRMS）进行非靶向脂质组学分析，全面鉴定从五种不同海洋鱼类中提取的脂质成分、红鲷鱼（Lutjanus campechanus (A1)）、大石斑鱼（Epinephelus lanceolatus (A2)）、巴浪鱼（Siganus canaliculatus (B2)）、白鲷鱼（Lates calcarifer (C3)）和鲣鱼（Katsuwonus pelamis (D2)）。采用 LC-HRMS 非靶向脂质组学分析方法，可在每种 MFO 中检测到 1000 多种正离子和负离子模式的脂质化合物。大多数脂质以甘油三酯（TG）和二甘油酯（DG）类为主。主成分分析（PCA）和偏最小二乘判别分析（PLS-DA）可将 MFO 样品分为正离子和负离子模式。根据预测的变量重要性（VIP）值，发现 15 种差异化脂质化合物在正离子模式下作为潜在的生物标记物具有重要作用。相反，在负电离模式下发现了 10 种差异化脂质化合物。由此可以得出结论，利用 LC-HRMS 进行非靶向脂质组学分析是全面鉴定 MFO 样品中脂质成分的一种强有力的分析技术。该技术还可进一步用于鉴定其他鱼油样品的脂质成分。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chromatographia 化学-分析化学

CiteScore

3.40

自引率

5.90%

发文量

103

审稿时长

2.2 months

期刊介绍： Separation sciences, in all their various forms such as chromatography, field-flow fractionation, and electrophoresis, provide some of the most powerful techniques in analytical chemistry and are applied within a number of important application areas, including archaeology, biotechnology, clinical, environmental, food, medical, petroleum, pharmaceutical, polymer and biopolymer research. Beyond serving analytical purposes, separation techniques are also used for preparative and process-scale applications. The scope and power of separation sciences is significantly extended by combination with spectroscopic detection methods (e.g., laser-based approaches, nuclear-magnetic resonance, Raman, chemiluminescence) and particularly, mass spectrometry, to create hyphenated techniques. In addition to exciting new developments in chromatography, such as ultra high-pressure systems, multidimensional separations, and high-temperature approaches, there have also been great advances in hybrid methods combining chromatography and electro-based separations, especially on the micro- and nanoscale. Integrated biological procedures (e.g., enzymatic, immunological, receptor-based assays) can also be part of the overall analytical process.